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A study on the structure -function relationship of radial glia cells

放射状胶质细胞结构与功能关系的研究

基本信息

批准号：
23570226
负责人：
ZHU YAN
金额：
$ 3.49万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2013
项目状态：
已结题

项目摘要

Radial glia cells are crucial to construct the nervous system. They have dual functions as neural progenitors as well as extending radial processes as physical scaffold for the developing neural tube. How their radial morphology is maintained and whether this is important are the key questions we have addressed in this project. Here we show that radial processes are disrupted in spinal cord of mouse mutants deficient in CXCL12/CXCR4/CXCR7 signaling. Interestingly this disruption causes leaky interface between the central nervous system and the periphery as cells from the latter are seen to invade into the former. We then showed that CXCL12 signaling affect the adhesiveness of radial glia to basement membrane components. Furthermore, we found that CXCL12 stimulation facilitates integrin B1 activation. Thus CXCL12/CXCR4/CXCR7 are important in maintaining the integrity of radial scaffold which in turn maintains the cellular segregation of the central nervous system.

放射状胶质细胞对神经系统的构建至关重要。它们具有双重功能，既可以作为神经祖细胞，也可以作为神经管发育的物理支架延伸放射状突起。它们的放射状形态是如何保持的，以及这是否重要是我们在这个项目中解决的关键问题。在这里，我们表明，放射状突起被破坏的CXCL 12/CXCR 4/CXCR 7信号转导缺陷的小鼠突变体的脊髓。有趣的是，这种破坏会导致中枢神经系统和外周神经系统之间的界面渗漏，因为可以看到来自后者的细胞侵入前者。然后，我们发现CXCL 12信号影响放射状胶质细胞对基底膜成分的粘附。此外，我们发现CXCL 12刺激促进整合素B1活化。因此，CXCL 12/CXCR 4/CXCR 7在维持放射状支架的完整性方面是重要的，而放射状支架的完整性又维持中枢神经系统的细胞分离。