Novel label-free tool for infections diagnosis based on Nano-Electro Optical Tweezers

基于纳米电光镊的新型无标签感染诊断工具

基本信息

项目摘要

I studied theoretically the enhancement of the electromagnetic field of the device using Comsol Multiphysics software in my third year of the project. I have designed and fabricated the size of the nano-aperture comparable to the volume of a single-bacterium so that the microorganism flowing close to the region with a strong field will be trapped, permitting studies at the single-cell level. When the trap was full, there is not energy confined around the trapped bacterium, so preventing, or at least remarkably reducing the probability, that another bacterium will be trapped in the same region. Moreover, by introducing an array of nanohole, many trapping sites have be activated at the same time; hence the proposed approach enabled the simultaneous analysis of several bacteria locally in well-defined positions. By arraying the nanohole, multiple signatures in parallel will be obtained, permitting a measurable signal in case of very weak interaction.I found the theoretical resonance modes of the proposed device. Using antibiotics added to the surrounding medium, I have examined the growth, survival, and reproduction mechanisms of the trapped bacteria using Raman spectroscopy. Unfortunately, the results are not good. As a result, I analyzed the SERS signal during the growth stages of the bacteria. In my study, I found that the intensity of the amide protein characteristics peaks increases during exponential phases and decreases during stationary phases. The results are in agreement with the theoretical explanations.
在项目的第三年,我使用Comsol Multiphysics软件对设备的电磁场增强进行了理论研究。我已经设计并制造了与单个细菌体积相当的纳米孔径大小,以便在强磁场区域附近流动的微生物将被捕获,从而允许在单细胞水平上进行研究。当陷阱被填满时,被困细菌的周围就没有能量了,因此可以防止,或者至少显著降低另一个细菌被困在同一区域的可能性。此外,通过引入纳米孔阵列,可以同时激活多个捕获位点;因此,所提出的方法能够同时分析几种细菌在明确的局部位置。通过排列纳米孔,将获得并行的多个特征,在非常弱的相互作用下允许可测量的信号。我找到了这个装置的理论共振模式。使用添加到周围培养基中的抗生素,我使用拉曼光谱检查了被捕获细菌的生长,生存和繁殖机制。不幸的是,结果并不好。因此,我分析了细菌生长阶段的SERS信号。在我的研究中,我发现酰胺蛋白特征峰的强度在指数阶段增加,在平稳阶段减少。结果与理论解释一致。

项目成果

期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Giant optical forces using an array of asymmetric split-ring plasmonic nanostructures
使用不对称裂环等离子体纳米结构阵列的巨大光学力
  • DOI:
  • 发表时间:
    2020
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Domna Kotsifaki;Viet Giang Truong;Sile Nic Chormaic
  • 通讯作者:
    Sile Nic Chormaic
Plasmonic Fano-resonant metamaterial for nanoparticle trapping and biosensing (invited talk):
用于纳米颗粒捕获和生物传感的等离子法诺共振超材料(特邀演讲):
  • DOI:
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    0
  • 作者:
    T. Nagaoka;T. Kozuka;T. Yamada;H. Habe;M. Nemoto;M. Tada;K. Abe;H. Handa;H. Yoshida;K. Ishii;Y. Kimura;Domna G. Kotsifaki
  • 通讯作者:
    Domna G. Kotsifaki
Fano resonant-assisted optical tweezers for dynamic multiple nanoparticle trapping
用于动态多纳米颗粒捕获的法诺共振辅助光镊
  • DOI:
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    0
  • 作者:
    D Kotsifaki;VG Truong;S Nic Chormaic
  • 通讯作者:
    S Nic Chormaic
Analysis of small plastics in coastal surface water samples of Okinawa using optical tweezers-Raman spectroscopy
利用光镊-拉曼光谱分析冲绳沿海地表水样中的小塑料
  • DOI:
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    0
  • 作者:
    D Kotsifaki;C Ripken and S Nic Chormaic
  • 通讯作者:
    C Ripken and S Nic Chormaic
Manipulation, trapping, and biosensing using metamaterial devices
使用超材料设备进行操纵、捕获和生物传感
  • DOI:
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Takano T;Omiya Y;Endo K;Oya M;Tano K;Fukuda Y;Matsumoto M;Takano S;Tokuno S;Domna G. Kotsifaki
  • 通讯作者:
    Domna G. Kotsifaki
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KOTSIFAKI Domna其他文献

KOTSIFAKI Domna的其他文献

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Lensfree On-Chip Near-field Microscopy based on Resonant Nano-Apertures
基于共振纳米孔径的无透镜片上近场显微镜
  • 批准号:
    7574253
  • 财政年份:
    2008
  • 资助金额:
    $ 2.58万
  • 项目类别:
Lensfree On-Chip Near-field Microscopy based on Resonant Nano-Apertures
基于共振纳米孔径的无透镜片上近场显微镜
  • 批准号:
    7694293
  • 财政年份:
    2008
  • 资助金额:
    $ 2.58万
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