Lensfree On-Chip Near-field Microscopy based on Resonant Nano-Apertures

基于共振纳米孔径的无透镜片上近场显微镜

• 批准号: 7574253
• 负责人: Aydogan Ozcan
• 金额: $ 17.45万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-30 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7574253
• 关键词: Algorithms; Area; Biology; Blood specimen; CD4 Positive T Lymphocytes; Cell Count; Cellular Phone; Chemistry; Computers; Condition; Count; Data; Developed Countries; Developing Countries; Development; Devices; Diagnostic; Electronics; Erythrocytes; Genomics; Health Personnel; Healthcare; Histopathology; Image; Imaging technology; Internet; Lighting; Measurement; Mechanics; Medical; Medicine; Metals; Microfluidic Microchips; Microfluidics; Microscope; Microscopy; Modality; Nanoarray Analytical Device; Optics; Patients; Physicians; Positioning Attribute; Property; Proteomics; Public Health; Purpose; Research; Resolution; Resources; Sampling; Scanning; Scheme; Speed; Standards of Weights and Measures; Surface; System; Telepathology; Testing; Time; Today; Training; Whole Blood; base; cell type; concept; cost; design; digital; high throughput screening; improved; interest; lens; miniaturize; nano; near field microscopy; novel; photonics; point of care; sensor; transmission process; two-dimensional

DESCRIPTION (provided by applicant): To achieve high spatial resolution, most optical microscopes rely on expensive components such as high numerical aperture objectives or other lenses. Due to such free-space components, most high resolution microscopes still remain bulky, making them harder to integrate with cost-effective and miniaturized systems. However, in medicine/biology there exists a set of applications that would highly benefit from miniaturized high resolution microscopes that are ideally on chip. For this purpose, the proposed research plan aims to develop a new form of lens-free, compact digital microscope using a densely packed array of on chip nano-apertures. By properly designing an array of metallic nano-apertures, a high spatial resolution will be achieved even without using any lens or mechanical scanning. For this end, the designed nano-array will be fabricated onto a standard opto-electronic sensor array, and the object of interest will be in direct contact with the aperture array. As a result, the proposed on chip microscope can image two dimensional objects (>5 mm2 wide) at a high spatial resolution of d500 nm, and with a fast imaging speed of ~150 mm2/min. Overall, this application aims to advance the current state of on chip microscopy by using the key concepts of nano-photonics and plasmonics. Because the proposed approach is lensfree, it can be integrated with microfluidic systems in a compact space, and thus can significantly improve the way that high-throughput screening microscopy or point-of-care diagnostics are currently done. High resolution and rapid imaging technologies that can be integrated with disposable microfluidic devices are urgently needed today, especially for the developing world, where the resources are scarce. Another significant impact of the proposed imaging modality in health care may be in the field of histopathology. The proposed near-field imaging scheme can potentially enable quite rapid capturing of high resolution images of histopathology samples, which can then be transmitted over the internet to any physician in the world. This opportunity, which is termed "telepathology", will imply the global sharing of medical resources. For this end, this application may enable practical implementations of telepathology by significantly improving the imaging speed (>150 mm2/min). This rapid image acquisition speed may also have a significant impact on micro-array based genomics or proteomics, where massive amounts of high resolution data need to be collected in a short amount of time. PUBLIC HEALTH RELEVANCE: Lensfree On-Chip Near-field Microscopy based on Resonant Nano-Apertures Project Narrative We propose to develop a novel high resolution and lens-free digital microscope that is based on optical resonance properties of a specially designed array of nano-apertures fabricated on an opto-electronic sensor chip. This new microscopy approach does not require any mechanical scanning or objective-lenses, and therefore offers a simpler and more compact approach for high resolution near-field imaging. Furthermore, the image acquisition and computation time is fairly short, making it a quite fast imaging modality that can significantly improve the way that high-throughput screening microscopy or point-of-care diagnostics are currently done.

描述（由申请人提供）：为了实现高空间分辨率，大多数光学显微镜依赖于昂贵的组件，如高数值孔径物镜或其他透镜。由于这些自由空间组件，大多数高分辨率显微镜仍然体积庞大，使它们难以与具有成本效益和小型化的系统集成。然而，在医学/生物学中，有一组应用将从理想的芯片上的小型化高分辨率显微镜中受益匪浅。为此，提出的研究计划旨在开发一种新型的无透镜、紧凑的数字显微镜，使用芯片上密集排列的纳米孔径阵列。通过适当设计金属纳米孔阵列，即使不使用任何透镜或机械扫描，也可以获得高空间分辨率。为此，设计的纳米阵列将被制造到一个标准的光电传感器阵列上，并且感兴趣的对象将与孔径阵列直接接触。结果表明，所设计的片上显微镜能够以d500 nm的高空间分辨率对宽约5 mm2的二维物体进行成像，成像速度可达150 mm2/min。总的来说，本应用程序旨在通过使用纳米光子学和等离子体动力学的关键概念来推进芯片上显微镜的现状。由于所提出的方法是无透镜的，它可以与微流体系统集成在一个紧凑的空间中，因此可以显著改善目前高通量筛选显微镜或即时诊断的方式。可以与一次性微流控装置集成的高分辨率和快速成像技术是当今迫切需要的，特别是对于资源稀缺的发展中国家。提出的成像模式在医疗保健中的另一个重要影响可能是在组织病理学领域。所提出的近场成像方案可以潜在地快速捕获组织病理学样本的高分辨率图像，然后可以通过互联网传输给世界上的任何医生。这个被称为“心灵病理学”的机会将意味着医疗资源的全球共享。为此，该应用程序可以通过显着提高成像速度（>150 mm2/min）来实现心灵病理学的实际实现。这种快速的图像采集速度也可能对基于微阵列的基因组学或蛋白质组学产生重大影响，其中需要在短时间内收集大量高分辨率数据。