SCN2A自閉症に関する膜電位イメージングによる細胞体樹状突起シグナリングの解析

通过膜电位成像分析 SCN2A 自闭症的体细胞树突信号传导

• 批准号: 21F20712
• 负责人: Kuhn Bernd
• 金额: $ 0.77万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-09-28 至 2023-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21F20712/
• 关键词: Scn2a/Nav1.2; neurological disorders; In vivo calcium imaging

Established a novel mouse model to study disease mechanisms underlying Scn2a neurodevelopmental disorders. For the first time, in vivo two-photo calcium imaging was performed in a Scn2a mouse model. We found that single neuron activities, as well as population co-activities were reduced in Scn2a insufficient mice during wakefulness. This may be one of the pathological mechanisms driving Scn2a neurodevelopmental disorders. Results was presented in the Japan neuroscience Society Annual Meeting and in the Okinawa Institute of Science and Technology (OIST) Internal Seminar.A manuscript is in preparation and will be submitted to a peer reviewed journal.Established collaboration opportunities between OIST and Florey Institute of Neuroscience and Mental Health, Australia.

建立一种新的小鼠模型来研究Scn2a神经发育障碍的疾病机制。首次在Scn2a小鼠模型中进行体内双光钙成像。我们发现，在清醒状态下，Scn2a不足的小鼠的单个神经元活动以及群体共同活动都减少了。这可能是Scn2a神经发育障碍的病理机制之一。研究结果在日本神经科学学会年会上和冲绳科学技术研究所（OIST）内部研讨会上发表。手稿正在准备中，将提交给同行评议的期刊。OIST与澳大利亚弗洛里神经科学和心理健康研究所建立了合作机会。

项目成果

Examining the in vivo neural network activity of a Scn2a loss-of-function mouse model

检查 Scn2a 功能丧失小鼠模型的体内神经网络活动

• 发表时间: 2022
• 作者: 濱田健太郎;篠崎陽一;行方和彦;大野伸彦;原田高幸;柏木賢治;小泉修一;Melody Li
• 通讯作者: Melody Li