Regulatory functions of chloroplast-associated non-coding RNAs in acclimation processes (C03)

叶绿体相关非编码RNA在适应过程中的调节功能（C03）

• 批准号: 317555487
• 金额: --
• 依托单位: Professur für Molekulare Zellbiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/317555487?language=en
• 关键词: Regulatory; functions; chloroplast; associated; non

We intend to uncover the high light and cold responsive small non-coding RNA (sRNA) repertoire in A. thaliana and to study their role in the regulation of genes encoding plastid-localised proteins. We will quantify levels of sRNAs and their cognate targets by RNA-seq in response to the high light and cold treatments that are centrally studied in the CRC/Transregio. We will also quantify sRNA and target RNA levels by RNA-seq in selected A. thaliana mutants with disordered retrograde signalling. By imposing perturbations to the identified plastid-related sRNA-regulated circuits we will examine their role in plant acclimation to high light and cold.

我们打算揭示高光和冷响应的小非编码RNA（sRNA）在A。thaliana，并研究它们在调控质体定位蛋白编码基因中的作用。我们将通过RNA-seq定量sRNA及其同源靶标的水平，以响应在CRC/Transregio中集中研究的强光和冷处理。我们还将通过RNA-seq在选定的A中定量sRNA和靶RNA水平。逆行信号紊乱的拟南芥突变体。通过对已鉴定的质体相关的sRNA调节回路施加扰动，我们将研究它们在植物适应强光和寒冷中的作用。