Characterizing physiological functions of metabolite transporters located in plastidic envelope membranes

表征位于质体包膜的代谢物转运蛋白的生理功能

• 批准号: 14540593
• 负责人: TANIGUCHI Mitsutaka
• 金额: $ 2.37万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14540593/
• 关键词: chloroplast; transporter; malate; 2-oxoplutarate; dicarboxylate; Arabidopsis thaliana; maize; rice

1.Analysis of Arabidopsis knockout and overexpressed plantsWe measured transport activities of chloroplasts isolated from knockout plants and found that the transport of malate and glutamate is facilitated mainly by AtpDCT1. Moreover, it was revealed that oxaloacetate transport is mediated only by AtpDCT1 and AtpOMT1. We also produced overexpression lines for OMT or DCT genes, and are comparing their photochemical electron transport activities and chloroplast microstructure under stress conditions to investigate the relationship of OMT and DCT to carbon/nitrogen assimilation and malate valve.2.Analysis of maize OMT and DCTsWe isolated four cDNAs, ZmpOMT1 and ZmpDCT1 to 3,from maize, a NADP-malic enzyme-type C_4 plant. We concluded that these OMT and DCTs function in the C_4 photoshynthetic pathway : ZmpOMT1 participates in the import of oxaloacetate into mesophyll chloroplasts in exchange for stromal malate and ZmpDCT2/3 could import malate that is transported from mesophyll cells into bundle sheath cells.3.Analysis of rice OMT and DCTsWe identified one OMT gene (OspOMT1) and two DCT genes (OspDCT1,OspDCT2) from rice. Gene expression analyses suggested that a C_3 plant rice does not possess an abundant and cell-specific expression mechanism on its OMT and DCT genes in contrast to maize.

1.拟南芥敲除和过表达的植物的分析测量了从基因敲除植物中分离出的叶绿体的运输活性，发现苹果酸和谷氨酸的运输主要由ATPDCT1促进。此外，揭示了草乙酸转运仅由ATPDCT1和ATPOMT1介导。我们还为OMT或DCT基因产生了过表达的线，并在应力条件下比较了它们的光化学电子传输活性和叶绿体微观结构，以研究OMT和DCT与碳/氮的关系的关系。2。MAIZE OMT和DCTSWE分离的四个cdnas，ZMPOMT1和ZMPDCT1和ZMPDCT， NADP-MALIC酶型C_4植物。我们得出的结论是，这些OMT和DCT在C_4 Photohynthetic途径中起作用：ZMPOMT1参与将草乙酸进口到叶肉质叶绿体中，以换取基质的苹果酸和ZMPDCT2/3，可以从米肉囊池中识别蛋白酶的基因，并将其输入。 （OSPOMT1）和大米的两个DCT基因（OSPDCT1，OSPDCT2）。基因表达分析表明，与玉米相比，C_3植物水稻在其OMT和DCT基因上没有丰富的细胞特异性表达机制。

项目成果

谷口光隆: "プラスチドおよびミトコンドリアの代謝産物輸送"植物細胞工学別冊 植物細胞工学シリーズ18 植物の膜輸送システムポンプ・トランスポーター・チャネル研究の新展開(加藤潔,島崎研一郎,前島正義,三村徹郎編)(秀潤社). 106-118 (2003)

谷口光隆：《质体和线粒体代谢物转运》植物细胞工程专刊植物细胞工程系列18植物膜转运系统泵、转运蛋白和通道研究新进展（加藤清、岛崎健一郎、前岛正芳、三村哲郎编） ）（蜀军社）106-118（2003）。

Taniguchi, Y.et al.: "Differentiation of dicarboxylate transporters in mesophyll and bundle sheath chloroplasts of maize"Plant & Cell Physiology. 45. 187-200 (2004)

Taniguchi, Y.et al.：“玉米叶肉和束鞘叶绿体中二羧酸转运蛋白的分化”植物

Mitsuya, S. et al.: "Relationship between the distribution of Na and the damages caused by salinity in the leaves of rice seedlings grown under a saline condition"Plant Production Science. 5. 269-274 (2002)

Mitsuya, S. 等人：“盐分条件下生长的稻苗叶片中钠的分布与盐分造成的损害之间的关系”《植物生产科学》。

Taniguchi, Y. et al.: "Differentiation of dicarboxylate transporters in mesophyll and bundle sheath chloroplasts of maize"Plant & Cell Physiology. 45. 187-200 (2004)

Taniguchi, Y. 等人：“玉米叶肉和束鞘叶绿体中二羧酸转运蛋白的分化”植物

谷口光隆: "プラスチドおよびミトコンドリアの代謝産物輸送"植物細胞工学シリーズ18植物細胞で働くポンプ,トランスポーター,チャネル研究の新展開. 106-118 (2003)

Mitsutaka Taniguchi：“质体和线粒体中的代谢物运输”植物细胞工程系列 18 植物细胞中泵、转运蛋白和通道研究的新进展（2003 年）。