Characterizing physiological functions of metabolite transporters located in plastidic envelope membranes

表征位于质体包膜的代谢物转运蛋白的生理功能

• 批准号: 14540593
• 负责人: TANIGUCHI Mitsutaka
• 金额: $ 2.37万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14540593/
• 关键词: chloroplast; transporter; malate; 2-oxoplutarate; dicarboxylate; Arabidopsis thaliana; maize; rice

1.Analysis of Arabidopsis knockout and overexpressed plantsWe measured transport activities of chloroplasts isolated from knockout plants and found that the transport of malate and glutamate is facilitated mainly by AtpDCT1. Moreover, it was revealed that oxaloacetate transport is mediated only by AtpDCT1 and AtpOMT1. We also produced overexpression lines for OMT or DCT genes, and are comparing their photochemical electron transport activities and chloroplast microstructure under stress conditions to investigate the relationship of OMT and DCT to carbon/nitrogen assimilation and malate valve.2.Analysis of maize OMT and DCTsWe isolated four cDNAs, ZmpOMT1 and ZmpDCT1 to 3,from maize, a NADP-malic enzyme-type C_4 plant. We concluded that these OMT and DCTs function in the C_4 photoshynthetic pathway : ZmpOMT1 participates in the import of oxaloacetate into mesophyll chloroplasts in exchange for stromal malate and ZmpDCT2/3 could import malate that is transported from mesophyll cells into bundle sheath cells.3.Analysis of rice OMT and DCTsWe identified one OMT gene (OspOMT1) and two DCT genes (OspDCT1,OspDCT2) from rice. Gene expression analyses suggested that a C_3 plant rice does not possess an abundant and cell-specific expression mechanism on its OMT and DCT genes in contrast to maize.

1.拟南芥敲除和过表达植物的分析我们测量了从敲除植物中分离的叶绿体的转运活性，发现苹果酸和谷氨酸的转运主要由AtpDCT1促进。此外，研究表明草酰乙酸转运仅由 AtpDCT1 和 AtpOMT1 介导。我们还制作了OMT或DCT基因的过表达系，并比较了它们在胁迫条件下的光化学电子传递活性和叶绿体微观结构，以研究OMT和DCT与碳/氮同化和苹果酸阀的关系。2.玉米OMT和DCT的分析我们从玉米中分离了4个cDNA，ZmpOMT1和ZmpDCT1至3。 NADP-苹果酸酶C_4型植物。我们得出结论，这些OMT和DCT在C_4光合作用途径中发挥作用：ZmpOMT1参与将草酰乙酸导入叶肉叶绿体以换取基质苹果酸，而ZmpDCT2/3可以将从叶肉细胞转运的苹果酸导入束鞘细胞。 3.水稻OMT和DCT的分析我们鉴定了一个OMT基因 (OspOMT1) 和两个来自水稻的 DCT 基因 (OspDCT1,OspDCT2)。基因表达分析表明，与玉米相比，C_3植物水稻的OMT和DCT基因不具有丰富的细胞特异性表达机制。

项目成果

谷口光隆: "プラスチドおよびミトコンドリアの代謝産物輸送"植物細胞工学別冊 植物細胞工学シリーズ18 植物の膜輸送システムポンプ・トランスポーター・チャネル研究の新展開(加藤潔,島崎研一郎,前島正義,三村徹郎編)(秀潤社). 106-118 (2003)

谷口光隆：《质体和线粒体代谢物转运》植物细胞工程专刊植物细胞工程系列18植物膜转运系统泵、转运蛋白和通道研究新进展（加藤清、岛崎健一郎、前岛正芳、三村哲郎编） ）（蜀军社）106-118（2003）。

Taniguchi, Y.et al.: "Differentiation of dicarboxylate transporters in mesophyll and bundle sheath chloroplasts of maize"Plant & Cell Physiology. 45. 187-200 (2004)

Taniguchi, Y.et al.：“玉米叶肉和束鞘叶绿体中二羧酸转运蛋白的分化”植物

Taniguchi, Y. et al.: "Differentiation of dicarboxylate transporters in mesophyll and bundle sheath chloroplasts of maize."Plant Cell Physiol.. 45. 187-200 (2004)

Taniguchi, Y. 等人：“玉米叶肉和束鞘叶绿体中二羧酸转运蛋白的分化。”Plant Cell Physiol.. 45. 187-200 (2004)

谷口光隆: "プラスチドおよびミトコンドリアの代謝産物輸送"植物細胞工学シリーズ18 植物細胞で働くポンプ,トランスポーター,チャネル研究の新展開. (印刷中). (2003)

Mitsutaka Taniguchi：“质体和线粒体中的代谢物运输”植物细胞工程系列 18 植物细胞中泵、转运蛋白和通道的研究新进展（2003 年）。

Kawasaki, M. et al.: "Structural and immunocytochemical characterization of the synthesis and accumulation of starch in sweet potato (Ipomoea batatas Lam.) tuberous root"Plant Production Science. 5. 152-159 (2002)

Kawasaki, M. 等人：“甘薯 (Ipomoea batatas Lam.) 块根淀粉合成和积累的结构和免疫细胞化学特征”植物生产科学。