Transcriptional and epigenetic programs of phenotypic plasticity to heatwave in reef fish

利用长读测序和染色体构象捕获技术，我们组装并注释了高质量的假小丑鱼（Amphiprion ocellaris）染色体规模的基因组，这是研究气候变化和生态的新兴模式动物。基因组被26,797个蛋白质编码基因注释。我们还鉴定出72个仅在斑蝽及其姊妹种佩cula分支中保守的基因。这些基因与神经系统功能有关，可能表明涉及特定分化机制的遗传工具包。我们还获得了经夏季高温处理的斑蝽F1代幼虫的综合转录组，这将作为显示热胁迫下细胞活动的遗传资源。

We assembled and annotated a highest-quality chromosome-scale genome of the false clownfish Amphiprion ocellaris, an emerging model animal for studying the climate change and ecology using long-read sequencing and chromosome conformation capture techniques. The genome was annotated with 26,797 protein-coding genes. We also identified 72 genes conserved only in A. ocellaris and sister species A. percula branch. These genes are related to nervous system functions potentially indicating the genetic toolkits involved in specific divergence mechanisms.We also generated comprehensive larval transcriptomes of F1 generation of A. ocellaris treated with high summer temperature, which will serve as the genetic resources showing the cellular activities during heat stress.