High resolution structure characterisation and stabilisation of supramolecular architectures using novel perfluoralkyl functionalised dendron-amphiphiles with dye spacers as optical probes

使用新型全氟烷基官能化树枝状两亲物和染料间隔基作为光学探针进行超分子结构的高分辨率结构表征和稳定

• 批准号: 317551446
• 负责人: Privatdozent Dr. Christoph Böttcher
• 金额: --
• 依托单位: Abteilung Organische Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/317551446?language=en
• 关键词: resolution; structure; characterisation; stabilisation; supramolecular

Supramolecular assemblies can be characterised in their quasi native state on the nano scale using cryogenic transmission electron microscopy (cryo-TEM), but an exact determination of the molecular organisation is achieved in only rare cases. If utilising particular cyanine dyes as an integrated component of an amphiphilic self-assembling systems, their optical properties, which alter in a specific way upon aggregation (e.g. by the formation of H- and J-aggregates), can, however, be used for a complementary determination of the molecular arrangement within the nanoscopic framework. Consequently, crucial parameters for an understanding of the self-organisation process on the molecular level are available.On the basis of these findings, we propose a new type of amphiphilic molecules in order to (a) characterise their self-assembled architectures, (b) produce photostable supramolecular aggregates, and (c) establish diagnostic transporters which are capable of self-signalling the release of their payloads. Our approach combines the particular properties of dendritic polyglycerol head groups of differing generations (G1-G3) and the optical properties of cyanine dye probes. In addition, alkyl chains as well as fluorinated alkyl chains will be introduced and their structure forming properties be characterised by spectroscopy and direct imaging techniques, in particular Cryo-TEM or Cryo-Electron Tomography. The combined efficacy of the hydrophobic effect, directional properties of dendritic head groups, the aggregation tendency of dye systems, as well as compartimentalisation effect (microphase separation) of fluorinated chains aims at the creation of particularly stable and structurally defined ultrastructures, which are applicable as specialised transporters due to their enhanced photostability and optical properties.

可以使用低温透射电子显微镜（Cryo-TEM）以纳米尺度上的准天然状态来表征超分子组件，但仅在极少数情况下才能实现分子组织的精确测定。如果将特定的氰染料用作两亲性自组装系统的集成组成部分，则其光学特性（例如，在聚集时会以特定方式改变（例如，通过形成H-和J-Aggregates），可以用于互补的纳疗框架内分子布置的互补测定。因此，可以使用这些发现的基础，我们提出了一种新型的两亲性分子，以（a）表征他们的自组成的建筑，（b）可以释放出来的诊断型诊断，我们提出了一种新型的自我组成的型结构，以理解分子水平上的自我组织过程的关键参数。我们的方法结合了不同世代（G1-G3）的树突状聚甘油头组和氰氨基染料探针的光学特性。此外，还将引入烷基链以及氟化烷基链，并以光谱和直接成像技术（尤其是冷冻TEM或冷冻电子层析成分）为特征。疏水作用，树突头组的方向性，染料系统的聚集趋势以及氟化链的局限性效应（微量年分离）的综合疗效旨在创建特别稳定的结构性和结构定义的紫外线结构，这是由于其专用迁移术的适用性，并且由于其优异的光构和具有选择性的光构成。

项目成果

Stereochemistry‐Controlled Supramolecular Architectures of New Tetrahydroxy‐Functionalised Amphiphilic Carbocyanine Dyes

• DOI: 10.1002/chem.201905745
• 发表时间: 2020-02
• 期刊: Chemistry (Weinheim an Der Bergstrasse, Germany)
• 作者: B. Schade;A. Singh;Virginia Wycisk;J. L. Cuéllar-Camacho;H. Berlepsch;R. Haag;C. Böttcher