Cross talk between a conjugative plasmid and its Bacillus subtilis host cell during stress adaptation (E02*)

应激适应过程中接合质粒与其枯草芽孢杆菌宿主细胞之间的串扰 (E02*)

• 批准号: 317946759
• 金额: --
• 依托单位: Zentrum für Synthetische Mikrobiologie (SYNMIKRO)
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/317946759?language=en
• 关键词: Cross; talk; between; conjugative; plasmid

Conjugative plasmid pLS20 affects many cellular processes and changes the transcription of about 5% of the genes of its host, and confers considerable stress resistance during active conjugation. Conversely, the host cell affects the timing of conjugation, which is centrally regulated by a plasmid-encoded repressor controlled by a quorum-sensing Rap/Phr system. We will analyse how the cross talk between plasmid and chromosome occurs on a molecular genetic and biochemical level and how the plasmid/host systems adapt to different stress conditions.

接合质粒 pLS20 影响许多细胞过程并改变其宿主约 5% 基因的转录，并在主动接合过程中赋予相当大的抗逆性。相反，宿主细胞影响接合时间，该时间由群体感应 Rap/Phr 系统控制的质粒编码阻遏物集中调节。我们将分析质粒和染色体之间的串扰如何在分子遗传和生化水平上发生，以及质粒/宿主系统如何适应不同的胁迫条件。