Cross talk between a conjugative plasmid and its Bacillus subtilis host cell during stress adaptation (E02*)

偶联质粒pLS20影响许多细胞过程，改变宿主约5%基因的转录，并在主动偶联过程中具有相当大的抗逆性。相反，宿主细胞影响偶联的时间，这是由群体感应Rap/Phr系统控制的质粒编码抑制因子集中调节的。我们将分析质粒和染色体之间的串扰如何在分子遗传和生化水平上发生，以及质粒/宿主系统如何适应不同的胁迫条件。

Conjugative plasmid pLS20 affects many cellular processes and changes the transcription of about 5% of the genes of its host, and confers considerable stress resistance during active conjugation. Conversely, the host cell affects the timing of conjugation, which is centrally regulated by a plasmid-encoded repressor controlled by a quorum-sensing Rap/Phr system. We will analyse how the cross talk between plasmid and chromosome occurs on a molecular genetic and biochemical level and how the plasmid/host systems adapt to different stress conditions.