Evolution and functional characterization of virulent strain-specific genes in Colletotrichum

炭疽菌毒力菌株特异性基因的进化和功能特征

• 批准号: 19K15846
• 负责人: GAN PAMELA
• 金额: $ 2.75万
• 依托单位: Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-04-01 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19K15846/
• 关键词: Genome rearrangement; cluster; virulence; fungal genome; anthracnose; nudix; effector; Keywords; fungi; genome; evolution; Colletotrichum; pathogen; Pathogen; Evolution; Fungi; Effector

Understanding how virulence emerges in pathogens can contribute to the development of better methods of disease control. We discovered a virulent strain-specific cluster of genes in Colletotrichum fungi which cause strawberry anthracnose that has an uneven phylogenetic distribution across multiple species. In FY2020, the following was achieved:1. Submitted paper describing potential virulence gene clusters in sub-telomeric regions and repeat-rich chromosomes. The work and associated genome and transcriptome data has been publicly released on NCBI, github and Biorxiv and is currently under review in the journal Genome Research.2. One of the proteins present in the potential virulence gene cluster, CfNudix, was characterized further. Phylogenetic analyses of all C. fructicola nudixes including CfNudix was carried out to determine the most closely related sequences to identify potential substrates by homology to previously characterized nudixes. The substrate specificity of purified CfNudix was tested on known nudix substrates.

了解病原体的毒力如何产生有助于开发更好的疾病控制方法。我们在炭疽菌真菌中发现了一个毒力强的菌株特异性基因簇，该基因簇在多个物种中具有不均匀的系统发育分布。2020财年，主要实现以下目标：提交的论文描述了亚端粒区和富含重复的染色体中潜在的毒力基因簇。这项工作以及相关的基因组和转录组数据已在NCBI、github和Biorxiv上公开发布，目前正在《基因组研究》杂志上进行审查。潜在毒力基因簇中存在的一种蛋白质CfNudix进一步被表征。对包括CfNudix在内的所有fruticola ndixes进行系统发育分析，确定最密切相关的序列，通过与已有鉴定的ndixes同源性鉴定潜在的底物。纯化后的CfNudix在已知的nudix底物上进行底物特异性测试。

项目成果

Genome rearrangements drive the evolution of virulence-related genes in the genomes of Colletotrichum gloeosporioides species complex

基因组重排驱动胶孢炭疽菌物种复合体基因组中毒力相关基因的进化

• 发表时间: 2019
• 作者: Gan P;Hiroyama R;Tsushima A;Masuda S;Kumakura N;Hoat TX;Suzuki T;Narusaka M;Narusaka Y;Takano Y;Shirasu K
• 通讯作者: Shirasu K

High-Quality Draft Genome Sequence of Fusarium oxysporum f. sp. cubense Strain 160527, a Causal Agent of Panama Disease

• DOI: 10.1128/mra.00654-19
• 发表时间: 2019-07-01
• 期刊: MICROBIOLOGY RESOURCE ANNOUNCEMENTS
• 影响因子: 0.8
• 作者: Asai, Shuta;Ayukawa, Yu;Arie, Tsutomu
• 通讯作者: Arie, Tsutomu

Colletotrichum shisoi sp. nov., an anthracnose pathogen of Perilla frutescens in Japan: molecular phylogenetic, morphological and genomic evidence

• DOI: 10.1038/s41598-019-50076-5
• 发表时间: 2019-09-16
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Gan, P.;Tsushima, A.;Shirasu, K.
• 通讯作者: Shirasu, K.