Functional modulation of the K+ transporter KtrAB in vivo and in vitro (P22*)

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The KtrAB system is a key player for the K+ homeostasis in bacteria. Though its role in bacteria is well described we are lacking knowledge on the molecular function and regulation of the complex. In this project we will focus on the role of different stimuli, namely nucleotide binding and the modulation by the plasma membrane composition. In vivo studies using fluorescence microscopy will elucidate the protein assembly and distribution in osmotically stressed and non-stressed cells. In vitro, structural and dynamic information is gained from cryo-EM, X-ray crystallography and EPR spectroscopy while the proteins¿ function is studied by electrophysiology and transport assays.