Functional modulation of the K+ transporter KtrAB in vivo and in vitro (P22*)

K 转运蛋白 KtrAB 体内和体外的功能调节 (P22*)

• 批准号: 319163479
• 金额: --
• 依托单位: Institut für Biochemie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/319163479?language=en
• 关键词: Functional; modulation; K+; transporter; KtrAB

The KtrAB system is a key player for the K+ homeostasis in bacteria. Though its role in bacteria is well described we are lacking knowledge on the molecular function and regulation of the complex. In this project we will focus on the role of different stimuli, namely nucleotide binding and the modulation by the plasma membrane composition. In vivo studies using fluorescence microscopy will elucidate the protein assembly and distribution in osmotically stressed and non-stressed cells. In vitro, structural and dynamic information is gained from cryo-EM, X-ray crystallography and EPR spectroscopy while the proteins¿ function is studied by electrophysiology and transport assays.

KtrAB系统是细菌K+稳态的关键参与者。虽然它在细菌中的作用已经被很好地描述了，但我们对这种复合物的分子功能和调控还缺乏了解。在这个项目中，我们将重点研究不同刺激的作用，即核苷酸结合和质膜组成的调节。利用荧光显微镜的体内研究将阐明蛋白质在渗透胁迫和非胁迫细胞中的组装和分布。在体外，通过冷冻电镜、x射线晶体学和EPR光谱获得结构和动态信息，同时通过电生理和运输分析研究蛋白质的功能。