Well-designed shape anisotropical bi-metallic nanoparticles for optical bioanalytics

用于光学生物分析的精心设计的形状各向异性双金属纳米粒子

• 批准号: 320385762
• 负责人: Professor Dr. Wolfgang Fritzsche
• 金额: --
• 依托单位: Leibniz-Institut für Photonische Technologien (IPHT)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/320385762?language=en
• 关键词: Well; designed; shape; anisotropical; bi

Addressing pressing problems of modern medicine, biotechnology and environmental science, such as mastering of new and multiresistant pathogens, personalized medicine, or the development of sustainable biotechnological processes requires a diagnostic at the single cell level. Therefore novel and powerful sensoric approaches are needed, which combine a selective recognition with a general applicable transducing principle and a high transduction rate, and which are also applicable for small molecular ensembles or even single molecules. Optical sensing based on nanoscale transducer (LSPR localized surface plasmon resonance) shows a high potential for a broad application in bioanalytics with significant advantages (like simpler detection, miniaturization, parallelization) compared to the established SPR (propagating surface plasmon resonance). In order to utilize this potential, the proposed project develops novel, more sensitive plasmonic nanoparticles based on a combination of two sensitivity boosting effects, namely anisotropy (using silver prisms) and bi-metal composition. Anisotropic particles show an enhancement of the electromagnetic field in certain locations (like corners) leading to a higher sensitivity for refractive index changes by analytes binding there. This effect will be, for the first time, combined with the recently observed sensitivity enhancement due to thin secondary metal layer on plasmonic nanostructures [1].In order to utilize the advantages of such particles fully, a narrow distribution in size (and thereby in the resulting spectroscopic properties) is required, what will be realized by the development of a microfluidic synthesis of the particles. Beside more homogenous chemical and thermal seed formation conditions in small liquid compartments, this process is strongly influenced by the self-assembly of primary metal clusters into growing particle during the seed phase of the synthesis. Beyond increased primary seed formation, this microfludic synthetic approach enables to influence cluster arrangement independently from seed formation, what will allow the formation of novel bi-metallic particles with significantly improved biosensoric properties. Using form-anisotrope plasmonic nanoparticles, the proposed project will demonstrate how micro reaction technology can be applied for a systematic assembly of novel functional nanomaterials.

解决现代医学、生物技术和环境科学的紧迫问题，如掌握新的和多重耐药病原体、个性化医疗或可持续生物技术过程的发展，需要在单细胞水平上进行诊断。因此，需要新的和强大的传感器方法，其将选择性识别与普遍适用的转导原理和高转导率相结合，并且其也适用于小分子集合或甚至单个分子。与现有的表面等离子体共振（传播表面等离子体共振）相比，基于纳米级传感器（LSPR局部表面等离子体共振）的光学传感在生物分析中具有广泛应用的巨大潜力，具有显着优势（例如更简单的检测、小型化、并行化）。为了利用这一潜力，拟议的项目开发了新的，更敏感的等离子纳米粒子的基础上的两个灵敏度提升效果，即各向异性（使用银棱镜）和双金属组合物的组合。各向异性颗粒在某些位置（如角落）显示出电磁场的增强，导致对结合在那里的分析物的折射率变化具有更高的灵敏度。这种效应将首次与最近观察到的由于等离子体纳米结构上的薄第二金属层而引起的灵敏度增强相结合[1]。为了充分利用这种颗粒的优点，需要窄的尺寸分布（从而在所得的光谱特性中），这将通过开发颗粒的微流体合成来实现。除了更均匀的化学和热晶种形成条件下，在小的液体隔室，这个过程是强烈影响的自组装的初级金属簇成生长的颗粒在种子阶段的合成。 除了增加的初级晶种形成之外，这种微流体合成方法能够独立于晶种形成影响簇排列，这将允许形成具有显著改善的生物传感特性的新型双金属颗粒。使用形式各向异性等离子体纳米粒子，拟议的项目将展示如何微反应技术可以应用于新的功能纳米材料的系统组装。