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Quantifying cutin and wax deposition in developing apple fruit

量化苹果果实发育过程中的角质和蜡沉积

基本信息

批准号：
320667595
负责人：
Dr. Bishnu Prasad Khanal
金额：
--
依托单位：
Leibniz-Institut für Agrartechnik und Bioökonomie e.V. (ATB)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2016
资助国家：
德国
起止时间：
2015-12-31 至 2020-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/320667595?language=en
关键词：
Quantifying cutin wax deposition developing

项目摘要

Appearance determines the fresh market value in most fleshy fruit. Even cosmetic damage severely reduces the market value. Economically important examples include russeting and skin spot of apple. Both disorders are preceded by microscopic failure of the cuticular membrane (CM) resulting in impaired barrier function. Maintaining an intact CM requires a better understanding of cuticle formation in developing fruit. Recent studies suggest that continuous deposition of cutin and wax plays an important role in minimizing stress and strain in the CM of developing apple thereby minimizing the risk of CM failure. The objectives of our study are to (1) establish a system for quantifying rates of cutin and wax deposition in developing apple fruit using labeled precursors, (2) compare rates of deposition established using 13C-labeled precursors (stable isotopes) with those determined gravimetrically and (3) quantify any spatial radial heterogeneity in the deposition within the cuticle. In the first and second year, we will utilize 14C (in the lab) and 13C -labeled (in the field) C16 and C18 fatty acid precursors to quantify rates of cutin and wax deposition. In laboratory experiments the amount of radioactivity associated with the cutin and wax fraction is determined and treatment conditions leading to maximum incorporation are identified. In the field 13C incorporation will be quantified by mass spectrometry and compared with deposition rates determined gravimetrically. The third year will focus on potential spatial heterogeneity of cutin and wax deposition in developing fruit supplied with 13C fatty acid. The methods developed will be useful in studying the role of cutin and wax deposition in russet susceptibility of apple as affected by genotype and environmental factors.

外观决定了大多数肉质水果的新鲜市场价值。即使是外观损坏也会严重降低市场价值。经济上重要的例子包括苹果的赤锈病和皮斑病。这两种疾病之前都会出现角质膜（CM）的微观失效，导致屏障功能受损。保持完整的 CM 需要更好地了解发育中果实的角质层形成。最近的研究表明，角质和蜡的连续沉积对于最大限度地减少发育中苹果的 CM 中的应力和应变起着重要作用，从而最大限度地减少 CM 失败的风险。我们研究的目的是（1）建立一个系统，用于使用标记前体量化发育中的苹果果实中角质和蜡的沉积率，（2）将使用 13C 标记前体（稳定同位素）建立的沉积率与通过重量分析确定的沉积率进行比较，以及（3）量化角质层内沉积的任何空间径向异质性。在第一年和第二年，我们将利用 14C（在实验室）和 13C 标记（在现场）的 C16 和 C18 脂肪酸前体来量化角质和蜡沉积的速率。在实验室实验中，确定了与角质和蜡成分相关的放射性量，并确定了导致最大掺入的处理条件。在现场，13C 的掺入将通过质谱法进行量化，并与通过重量分析确定的沉积速率进行比较。第三年将重点研究开发含有 13C 脂肪酸的水果时角质和蜡沉积的潜在空间异质性。所开发的方法将有助于研究角质和蜡沉积在受基因型和环境因素影响的苹果赤褐色易感性中的作用。