Increasing methane productivity in anaerobic digesters by addition of CO2

通过添加 CO2 提高厌氧消化池中的甲烷生产率

• 批准号: 320889444
• 负责人: Privatdozent Dr.-Ing. Konrad Koch
• 金额: --
• 依托单位: Lehrstuhl für Siedlungswasserwirtschaft
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/320889444?language=en
• 关键词: Increasing; methane; productivity; anaerobic; digesters

The major goal of this project is the identification of the mechanisms leading to increased CH4 formation by the addition of CO2 during anaerobic digestion. While enough evidence of the effect has already been provided by various studies, the underlying mechanisms have only been hypothesized so far and are still far from being understood. In order to reproduce the effects and to utilize this great potential for energy production from waste streams, the mechanisms had to be fully uncovered. To achieve the above mentioned goals an interdisciplinary approach is required including the use of stable isotope methods as the key parameter for process identification, know-how of process engineering including the supply of a fully-equipped laboratory with expertise in the performance of continuous flow-through experiments, and accompanying microbial analyses. To identify the basic mechanisms of the observed bioconversion described in detail, we aim at establishing two continuous anaerobic digestion test systems at TUM. One system is enriched with CO2 while the other serves as the control. The reactors will be fed with sewage sludge, since it seems to be the substrate with the highest potential for CO2 enrichment. Tests will be carried out at various loading rates indicating different stress regimes which might have a significant influence on the mechanism of CH4-formation. During the tests, standard process parameters (TS, VS, COD, VFA, Alkalinity, pH value, gas quantity and quality) will be monitored. Additionally, the biocenosis in the digestate will be characterized by state-of-the-art molecular biological methods such as guild-specific quantitative real-time PCR on DNA and mRNA levels, and community sequencing approaches.As a special focus of the project, in order to retrace CO2 conversion to CH4, isotope techniques including isotope labeling experiments will be applied during the digestion tests. With this powerful technique it is possible to reconstruct precursors and specific pathways during CO2 addition.Once the underlying mechanisms are understood, the basis for implementing the approach in practice is provided.

该项目的主要目标是确定在厌氧消化过程中通过添加二氧化碳导致CH4形成增加的机制。虽然各种研究已经提供了足够的证据证明这种影响，但潜在的机制迄今为止只是假设，仍然远未被理解。为了再现这种效果并利用废物流产生能源的巨大潜力，必须充分揭示其机制。为了实现上述目标，需要一种跨学科的方法，包括使用稳定同位素方法作为工艺识别的关键参数，工艺工程的专有技术，包括提供设备齐全的实验室，具有连续流动实验的专业知识，以及伴随的微生物分析。为了确定详细描述的观察到的生物转化的基本机制，我们旨在在TUM建立两个连续厌氧消化测试系统。一个系统富含二氧化碳，而另一个系统作为控制。反应器将使用污水污泥，因为污泥似乎是CO2富集潜力最大的基质。试验将在不同的加载率下进行，表明可能对ch4形成机制产生重大影响的不同应力制度。在测试过程中，将监测标准工艺参数（TS， VS， COD， VFA，碱度，pH值，气体数量和质量）。此外，消化系统中的生物病将通过最先进的分子生物学方法进行表征，如行会特异性的DNA和mRNA水平实时定量PCR，以及社区测序方法。作为该项目的一个特别重点，为了追溯CO2转化为CH4，将在消化测试中应用同位素技术，包括同位素标记实验。利用这种强大的技术，可以重建二氧化碳添加过程中的前体和特定途径。一旦了解了潜在的机制，就为在实践中实现该方法提供了基础。