鉄イオンによるオキシム切断に基づく蛍光センサーによるリソソーム内鉄代謝機構の解明

在PD研究员（R3）的一年里，穆慧颖的研究还集中在设计和合成新型基于反应的功能性荧光传感器，以检测生物标本中与疾病相关的微小变化，如pH值和小分子。与她以前研究的花菁染料相比，半花菁染料具有功能化容易、合成步骤短等优点。因此，本论文通过简单的合成路线，引入具有不同供电子能力的取代基，开发了一系列pH响应型半菁染料。 在评估了它们的物理性质后，她发现这些传感器表现出可调的吸收和发射波长，斯托克斯位移和pH响应性取决于取代。其中，含有吗啉官能团作为溶酶体靶向部分的传感器显示出最佳的pKa值（6.2），用于选择性地在活细胞中进行亚细胞器成像。此外，该传感器在生物系统的刺激下，pH波动的检测表现出良好的可逆性。此外，本课题还将继续研究Fe（II）荧光探针的开发，并将在今后的研究中继续进行。

In the year of PD researcher (R3), Huiying Mu's research also focused on the design and synthesis of novel reaction-based functional fluorescent sensors to detect the diseases-related tiny changes in biological specimens such as pH, and small molecules. Compared with cyanine dyes in her previous research, the hemicyanine dyes have advantages in easy functionalization and short synthesis procedures. Therefore, a series of pH-responsive hemicyanine dyes were developed trough simple synthesized route to introduce the substitutions with varied electron donating ability. After evaluated their photophysical properties, she found that these sensors exhibited tunable absorption and emission wavelength, stokes shift, and pH-responsiveness depend on the substitutions. Among them, the sensor containing morphorine functional group as a lysosome targeting moiety showed optimized pKa value (6.2) for selectively subcellular organelle imaging in living cells. Furthermore, this sensor showed good reversibility for the detection of pH fluctuation under the stimulations under biological systems. The further research of this work will be continued for new applications in vivo.Besides,the authors' reasearch also foucused on the development of Fe(II)-responsive fluorescent probes, and this project will be continued in the future.