Optimization of production of antimicrobial substances from terrestrial cyanobacteria - from screnning to bioreactor development
陆生蓝藻抗菌物质生产的优化 - 从筛选到生物反应器开发
基本信息
- 批准号:323611467
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Grants
- 财政年份:2016
- 资助国家:德国
- 起止时间:2015-12-31 至 2020-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Multidrug-Resistant pathogens (MRP) are of major concern to hospitals today. The discovery and establishment of new antibiotics is therefore essential, along with a more conscious use of antibiotics. For this reason, the focus of this project is the cultivation of terrestrial cyanobacteria for the production of new antimicrobial substances. Antibacterial substances have previously been isolated from cyanobacteria and characterized; however there has been little research on terrestrial cyanobacteria due to the inability to optimally culture phototrophic biofilms. The Institute of Bioprocess Engineering at TU Kaiserslautern has access to one of the largest culture collections of terrestrial cyanobacteria (University of Kaiserslautern, Chair of Experimental Ecology, Prof. Dr. Büdel), and to the biosphere tower in the Biosphere Reserve Palatinate Forest/ Northern Vosges, which is located within an oak stand. This variety of potential antibiotic producers should be taken advantage of by testing cyanobacteria for their antibiotic effects and cultivating those showing positive results. A new emerse photobioreactor, developed at the Institute of Bioprocess Engineering, is ideal for the cultivation of those terrestrial cyanobacteria. The medium is vaporized by an ultrasound transducer and guided as an aerosol in the reactor. Drought stress and pH changes allow a maximum yield of extracellular polymeric substances (EPS) to be achieved while saving medium. In a biofilm system, antibiotics are not released into the media but accumulate in the EPS and can be isolated in higher concentration as in submerse systems. If antimicrobial activities are found, the structure of the related compounds must be elucidated. The initial titers of the active strains should be improved by varying different process parameters such as energy and carbon supply. Photoautotrophic, chemoorganotrophic and mixotrophic cultivations in shake flasks and in different bioreactor set-ups will be performed and antibiotic production will be analyzed. Finally, a process model should be investigated for antibiotic production by at least one cyanobacterium.
多药耐药病原体(MRP)是当今医院的主要关注点。 因此,发现和建立新的抗生素是必不可少的,沿着更有意识地使用抗生素。 因此,该项目的重点是培养陆地蓝藻,用于生产新的抗菌物质。抗菌物质以前已经从蓝藻中分离出来,并进行了表征;然而,由于不能最佳地培养光养生物膜,对陆生蓝藻的研究很少。 在TU Kaiseraustern生物过程工程研究所有机会获得陆地蓝藻最大的培养收藏之一(凯撒大帝大学实验生态学教授Büdel博士),以及位于普法尔茨森林/北方孚日生物圈保护区的生物圈塔,这种潜在的抗生素生产者应该通过测试蓝藻的抗生素效果来利用并培养那些表现出积极成果的人。 生物过程工程研究所研制的一种新型浮床光生物反应器是培养陆生蓝藻的理想设备。 该介质通过超声换能器蒸发并在反应器中作为气溶胶被引导。干旱胁迫和pH值的变化允许最大产量的胞外聚合物(EPS),同时节省介质。 在生物膜系统中,抗生素不会释放到培养基中,而是积累在EPS中,并且可以像浸没系统中那样以更高的浓度分离。 如果发现抗菌活性,必须阐明相关化合物的结构。 活性菌株的初始滴度应通过改变不同的工艺参数如能量和碳供应来提高。 将在摇瓶中和不同的生物反应器设置中进行光自养、化能有机营养和兼养培养,并分析抗生素产量。 最后,应该研究至少一种蓝细菌的抗生素生产过程模型。
项目成果
期刊论文数量(11)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Development of a lightweight multi-skin sheet photobioreactor for future cultivation of phototrophic biofilms on facades.
开发一种轻质多层板光生物反应器,用于未来在立面上培养光养生物膜
- DOI:10.1016/j.jbiotec.2020.06.004
- 发表时间:2020
- 期刊:
- 影响因子:4.1
- 作者:K. Scherer;J. Stiefelmaier;D. Strieth;M. Wahl;R. Ulber
- 通讯作者:R. Ulber
A new strategy for a combined isolation of EPS and pigments from cyanobacteria
- DOI:10.1007/s10811-020-02063-x
- 发表时间:2020-02-17
- 期刊:
- 影响因子:3.3
- 作者:Dorina, Strieth;Judith, Stiefelmaier;Roland, Ulber
- 通讯作者:Roland, Ulber
A qPCR method for distinguishing biomass from non-axenic terrestrial cyanobacteria cultures in hetero- or mixotrophic cultivations
- DOI:10.1007/s10811-020-02282-2
- 发表时间:2020-10
- 期刊:
- 影响因子:3.3
- 作者:J. Walther;A. Schwarz;M. Witthohn;D. Strieth;K. Muffler;R. Ulber
- 通讯作者:J. Walther;A. Schwarz;M. Witthohn;D. Strieth;K. Muffler;R. Ulber
Characterization of terrestrial phototrophic biofilms of cyanobacterial species
- DOI:10.1016/j.algal.2020.101996
- 发表时间:2020-09
- 期刊:
- 影响因子:5.1
- 作者:J. Stiefelmaier;D. Strieth;Sarah Di Nonno;N. Erdmann;K. Muffler;R. Ulber
- 通讯作者:J. Stiefelmaier;D. Strieth;Sarah Di Nonno;N. Erdmann;K. Muffler;R. Ulber
Terrestrische Cyanobakterien als Quelle für antimikrobielle Wirkstoffe
陆生蓝藻作为抗菌剂的来源
- DOI:10.1007/s12268-020-1484-1
- 发表时间:2020
- 期刊:
- 影响因子:0
- 作者:M. Witthohn;A. Schwarz;D. Strieth;S. Lenz;R. Ulber;K. Muffler
- 通讯作者:K. Muffler
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Professor Dr.-Ing. Kai Muffler其他文献
Professor Dr.-Ing. Kai Muffler的其他文献
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