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iPS神経変性疾患モデル－ロボット結合系を利用した経路探索機能再建の試み

iPS 神经退行性疾病模型 - 尝试使用机器人耦合系统重建寻路功能

基本信息

批准号：
20J22902
负责人：
張智翔
金额：
$ 1.98万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2020
资助国家：
日本
起止时间：
2020-04-24 至 2023-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20J22902/
关键词：
in vitro culture hippocampal neocortical time interval training human iPS neurons burst activity coupling HPC-CX coculture spatial-temporal memory electrical stimulation neurorobotic

项目摘要

Cell assemblies are essential for multiple brain functions, but damage to nodes within these assemblies can cause functional failure. It is hypothesized that activating remaining cell assemblies is essential to restore lost function. Activating pathways that bypass the damaged routes may be a potential method to achieve this. Electrical stimulation is a potential method to activate the bypassing pathway, given its potential to modify the synaptic connection between stimulated neurons.The research aimed to establish an in vitro platform to study how electrical stimulation can encode functions by modifying connections between neuronal assemblies. Two main results were obtained: establishing an in vitro neuronal system to study information transformation and evaluating the system to acquire information given by electrical stimulation programs.The developed system, composed of neocortical and hippocampal networks, demonstrated cross-frequency coupling properties via burst activities, which indicates the usage of the system in studying information transformation between multi-component neuronal systems, as published in Frontiers in Neuroscience.Using this system, a stimulation program called "time interval training" was developed to regulate the spiking relations between neurons, allowing for the adjustment of the burst activities propagation time between the two sub-cultures. Live imaging methods were then used to identify the participation of different neurons in burst events, providing valuable insight into the mechanism of the designed system for information possession.

细胞集合体对于多种大脑功能至关重要，但这些集合体内的节点受损可能导致功能衰竭。据推测，激活剩余的细胞组装体对于恢复失去的功能至关重要。激活绕过受损通路的通路可能是实现这一目标的潜在方法。电刺激是激活旁路通路的一种潜在方法，因为它有可能改变受刺激神经元之间的突触连接。该研究旨在建立一个体外平台，研究电刺激如何通过改变神经元组装体之间的连接来编码功能。获得了两个主要结果：建立了一个研究信息传递的体外神经元系统，并对该系统获取电刺激信息的能力进行了评价。该系统由皮层和海马网络组成，通过爆发活动表现出交叉频率耦合特性，表明该系统可用于研究多成分神经元系统之间的信息传递，使用该系统，开发了称为“时间间隔训练”的刺激程序来调节神经元之间的尖峰关系，允许调节两个亚培养物之间的爆发活动传播时间。然后使用实时成像方法来识别不同神经元在突发事件中的参与，从而为所设计的信息占有系统的机制提供有价值的见解。