Chromosome and protein dynamics during the insertion of membrane proteins into the cell membrane (P14)

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We will make use of single-molecule tracking to further describe the dynamics of mRNAs, ribo-somes, signal recognition particle (SRP) and of SRP receptor FtsY in Shewanella putrefaciens as Gram-negative model organism, and in Bacillus subtilis as Gram-positive organism. We will extend our studies to YidC and YlxM proteins, who function during membrane insertion, and to cold shock proteins, playing a role in mRNA localization. Changes in chromosome dynamics will be studied using superresolution fluorescence microscopy, employing computer modelling.