Chromosome and protein dynamics during the insertion of membrane proteins into the cell membrane (P14)

我们将利用单分子追踪技术进一步描述腐败希瓦氏菌（Shewanella putrefaciens）作为革兰氏阴性模式生物和枯草芽孢杆菌（Bacillus subtilis）作为革兰氏阳性生物中mRNA、核糖体、信号识别颗粒（SRP）和SRP受体FtsY的动态。我们将把我们的研究扩展到YidC和YlxM蛋白，它们在膜插入过程中发挥作用，以及冷休克蛋白，在mRNA定位中发挥作用。染色体动力学的变化将使用超分辨率荧光显微镜，采用计算机建模进行研究。

We will make use of single-molecule tracking to further describe the dynamics of mRNAs, ribo-somes, signal recognition particle (SRP) and of SRP receptor FtsY in Shewanella putrefaciens as Gram-negative model organism, and in Bacillus subtilis as Gram-positive organism. We will extend our studies to YidC and YlxM proteins, who function during membrane insertion, and to cold shock proteins, playing a role in mRNA localization. Changes in chromosome dynamics will be studied using superresolution fluorescence microscopy, employing computer modelling.