Interplay between fermentative and respiratory energy conservation in ruminal Prevotella sp.

瘤胃普雷沃氏菌发酵和呼吸能量守恒之间的相互作用。

• 批准号: 327953272
• 负责人: Professorin Dr. Julia Fritz-Steuber
• 金额: --
• 依托单位: Fachgebiet Funktionelle Mikrobiologie bei Nutztieren (460c)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/327953272?language=en
• 关键词: Interplay; between; fermentative; respiratory; energy

Prevotella is a predominant genus in the rumen microbiome, there they are important for non-cellulose polysaccharide and protein degradation. Because of their metabolic activities, Prevotella maintain the redox potential in the rumen and balance the rumen microbial activities. So far, Prevotella spp. were mainly studied because of their carbohydrate active enzymes and the proteolytic properties. Reductive pathways including the generation of NAD+ and the overall redox balance were ignored in the past. A preliminary study of the applicants identified the presence of Na+ -translocating NADH:quinone oxidoreductase (NQR) proteins affiliated to Prevotella spp. in rumen samples. Another analyses using a pure Prevotella bryantii culture verified the presence of NQR as the major oxidoreductase in the respiratory chain of this organism. This capacity can be coupled to the reduction of fumarate. The NQR is the target for the ionophore monensin, which is largely used to prevent ketosis in the cattle and to reduce methane emission. As Prevotella were almost not considered to be influenced by the ionophore treatment before, the presence of the detected NQR strongly indicates a possible negative effect. Thus, in the collaborative research project, we aim to understand how NADH:fumarate oxidoreduction catalysed by the Na+ -NQR and the fumarate reductase contributes to energy generation during fermentation of aspartate or glucose by P. bryantii (project 1, J. Steuber). The obtained knowledge is used to interpret proteomic and metabolomic data with pure Prevotella cultures and rumen samples (project 2, J. Seifert). Our long-term goal is a complete reconstruction of the catabolic reactions of these important ruminal bacteria and to identify possible agents, which inhibit their growth in the rumen and result in an imbalanced rumen microbiome with negative effects to the animal.

普雷沃氏菌是瘤胃微生物组中的主要属，它们对于非纤维素多糖和蛋白质降解很重要。由于其代谢活动，普雷沃氏菌维持瘤胃中的氧化还原电位并平衡瘤胃微生物活动。到目前为止，普雷沃氏菌属。主要因其碳水化合物活性酶和蛋白水解特性而被研究。过去忽略了包括 NAD+ 生成和整体氧化还原平衡在内的还原途径。申请人的初步研究确定了属于普雷沃菌属的 Na+ 转位 NADH:醌氧化还原酶 (NQR) 蛋白的存在。在瘤胃样品中。另一项使用纯布氏普氏菌培养物的分析证实了 NQR 是该生物体呼吸链中主要氧化还原酶的存在。这种能力可以与富马酸盐的减少相结合。 NQR 是离子载体莫能菌素的靶标，莫能菌素主要用于预防牛的酮症并减少甲烷排放。由于以前几乎不认为普雷沃菌受到离子载体处理的影响，因此检测到的 NQR 的存在强烈表明可能存在负面影响。因此，在合作研究项目中，我们旨在了解 Na+ -NQR 和富马酸还原酶催化的 NADH：富马酸氧化还原如何有助于 P. bryantii 发酵天冬氨酸或葡萄糖期间产生能量（项目 1，J. Steuber）。获得的知识用于解释纯普雷沃氏菌培养物和瘤胃样本的蛋白质组学和代谢组学数据（项目 2，J. Seifert）。我们的长期目标是完全重建这些重要的瘤胃细菌的分解代谢反应，并找出可能的药物，这些药物会抑制它们在瘤胃中的生长，导致瘤胃微生物组失衡，对动物产生负面影响。