Molecular and functional properties of the Na+ -translocating NADH:quinone oxidoreductase (Na+ -NQR) from Vibrio cholerae

霍乱弧菌 Na 转位 NADH:醌氧化还原酶 (Na -NQR) 的分子和功能特性

• 批准号: 212191952
• 负责人: Professorin Dr. Julia Fritz-Steuber
• 金额: --
• 依托单位: Fachgebiet Zelluläre Mikrobiologie (190i)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/212191952?language=en
• 关键词: Molecular; functional; properties; Na+; translocating

The bacterium Vibrio cholerae, the causative agent of Cholera disease, utilizes the Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) to generate a Na+ motive force. The Na+-NQR is a membrane-bound enzyme complex consisting of the six subunits NqrABCDEF. This redox-driven pump with a molecular mass of 220 kDa catalyzes the first step in the respiratory chain of V. cholerae and couples the exergonic oxidation of NADH to the endergonic translocation of Na+ from the cytoplasm to the periplasm. The mechanism of redox-driven Na+ transport by the Na+-NQR is unknown. Our long-term goal is to understand this process at atomic scale. To this end, we will analyze the electron transfer and Na+ translocation events using the solubilized and reconstituted pump, and characterize and crystallize subcomplexes for the determination of the 3D structure.

霍乱弧菌是引起霍乱的病原体，它利用Na+转运型NADH：苯醌氧化还原酶(Na+-NQR)产生Na+动力。Na+-NQR是一种膜结合酶复合体，由六个亚基NqrABCDEF组成。这个分子质量为220 kDa的氧化还原驱动泵催化霍乱弧菌呼吸链的第一步，并将NADH的外向氧化耦合到Na+从细胞质到周质的内源性转运。Na+-NQR氧化还原驱动Na+转运的机制尚不清楚。我们的长期目标是在原子尺度上理解这一过程。为此，我们将使用增溶和重组泵来分析电子转移和Na+转移事件，并表征和晶化亚络合物以确定3D结构。

项目成果

Role of the Na(+)-translocating NADH:quinone oxidoreductase in voltage generation and Na(+) extrusion in Vibrio cholerae.

Na( )-转位 NADH:醌氧化还原酶在霍乱弧菌电压产生和 Na( ) 挤出中的作用

• DOI: 10.1016/j.bbabio.2015.12.010
• 发表时间: 2016
• 期刊: Biochimica et biophysica acta
• 作者: Vorburger;Nedielkov;Brosig;Schunke;Steffen;Möller;Steuber
• 通讯作者: Steuber

Continuous fluorescence-based measurement of redox-driven sodium ion translocation.

基于连续荧光的氧化还原驱动钠离子易位测量

• DOI: 10.1016/j.ab.2014.05.012
• 发表时间: 2014
• 期刊: Analytical biochemistry
• 影响因子: 2.9
• 作者: Claussen;Karuppasamy;Schaffitzel;Steuber
• 通讯作者: Steuber

The structure of Na+-translocating of NADH:ubiquinone oxidoreductase of Vibrio cholerae: implications on coupling between electron transfer and Na+ transport

霍乱弧菌NADH泛醌氧化还原酶Na-转位的结构：对电子转移与Na传输耦合的影响

• DOI: 10.1515/hsz-2015-0128
• 发表时间: 2015
• 期刊: Biological Chemistry
• 影响因子: 3.7
• 作者: Steuber;Vohl G;Muras V;Toulouse C;Claußen B;Vorburger T;Fritz G.
• 通讯作者: Fritz G.

Structure of the V. cholerae Na+-pumping NADH:quinone oxidoreductase

• DOI: 10.1038/nature14003
• 发表时间: 2014-12-04
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Steuber, Julia;Vohl, Georg;Fritz, Guenter
• 通讯作者: Fritz, Guenter