Evolution of the Drosophila simulans proteome in response to adaptation to a novel temperature environment

模拟果蝇蛋白质组的进化以适应新的温度环境

• 批准号: 328697436
• 负责人: Dr. Kathrin Anna Otte
• 金额: --
• 依托单位: Institut für Populationsgenetik
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/328697436?language=en
• 关键词: Evolution; Drosophila; simulans; proteome; response

Understanding how organisms adapt to novel environments is one of the fundamental questions in evolutionary biology. Adaptation can be traced in the genome as allele frequency changes, which elicit downstream adaptive responses. Detailed knowledge of the molecular mechanisms underlying these adaptive responses is vital to connect the genotype to the phenotype. Temperature is a major environmental factor and affects various physiological and behavioral responses, especially in ectothermic animals like Drosophila. Adaptation to new thermal environments is detectable at multiple molecular levels, e.g. genome, transcriptome and proteome. The proposed project pioneers the use of proteomics in combination with experimental evolution to study proteins involved in temperature adaptation. Proteomics is especially suitable for characterizing molecular phenotypic responses, as proteins are the key molecules in almost all biological processes. We will study Drosophila simulans populations that have been exposed to two different thermal regimes for over 50 and over 100 generations, respectively. Genomic and transcriptomic resources available for these populations give us the unique opportunity to perform an integrated data analysis, studying adaptive responses in unprecedented detail to characterize causative genes and downstream targets of selection. We will investigate the following questions: 1. How does temperature adaptation affect protein abundance? 2. How does temperature adaptation affect the transcriptome and the proteome? 3. Does the integration of transcriptomic and proteomic data provide a better understanding of the molecular mechanisms underlying temperature adaptation? 4. Do transcriptomic and proteomic data improve the resolution of genomic signatures of adaptation?

了解生物体如何适应新环境是进化生物学的基本问题之一。适应性可以在基因组中追踪到等位基因频率的变化，从而引发下游的适应性反应。详细了解这些适应性反应的分子机制对于将基因型与表型联系起来至关重要。温度是一个主要的环境因素，影响着各种生理和行为反应，尤其是像果蝇这样的变温动物。对新的热环境的适应可以在多个分子水平上检测到，例如基因组、转录组和蛋白质组。该项目开创了将蛋白质组学与实验进化相结合的方法来研究与温度适应有关的蛋白质。蛋白质组学特别适用于表征分子表型反应，因为蛋白质是几乎所有生物过程的关键分子。我们将研究分别暴露在两种不同热环境下超过50代和100代的模拟果蝇种群。这些种群的基因组和转录组学资源为我们提供了进行综合数据分析的独特机会，以前所未有的细节研究适应性反应，以表征致病基因和下游选择目标。我们将调查以下问题：1。温度适应如何影响蛋白质丰度？2. 温度适应如何影响转录组和蛋白质组？3. 转录组学和蛋白质组学数据的整合是否能更好地理解温度适应的分子机制？4. 转录组学和蛋白质组学数据是否提高了适应基因组特征的分辨率？