One dimensional magnetic resonance spectroscopic imaging with radiofrequency field gradients/radiofrequency phase gradients and a microstrip as transmitter and receiver for the investigation of 3D cell cultures

使用射频场梯度/射频相位梯度和微带作为发射器和接收器的一维磁共振波谱成像，用于研究 3D 细胞培养物

• 批准号: 329207848
• 负责人: Dr. Jörg Lambert
• 金额: --
• 依托单位: Leibniz-Institut für Analytische Wissenschaften -ISAS- e.V.
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/329207848?language=en
• 关键词: dimensional; magnetic; resonance; spectroscopic; imaging

In the framework of this project we want to develop one dimensional nuclear magnetic resonance spectroscopic imaging techniques, which allow for the systematic investigation of the uptake of anticancer drugs by 3D cell culture systems. These techniques will allow for spatially resolved nuclear magnetic resonance measurements with high spatial and spectroscopic resolution for mass- and volume limited samples at any high resolution nuclear magnetic resonance spectrometer, without the need for any dedicated hardware. The distribution of metabolites and pharmaceutical drugs can be determined with this technique in 3D cell culture systems like spheroids as a function of time. The technique is based on a planar microstrip detector, which can easily handle microfluidic chips as sample holders to ensure the viability of the cells. The technique employs radiofrequency field and radiofrequency phase gradients for imaging to avoid mechanical interference and to reduce the influence of discontinuities of the susceptibility on the spectra. For the generation of radiofrequency field gradients the microstrip uses a triangular shaped structure, which gives rise to a linear gradient of current density and the corresponding radiofrequency field as a function of spatial displacement. A nutation technique is used to obtain spatial resolution. Based on this prototype a probe with radiofrequency phase gradients is to be developed, which contains two orthogonally arranged microstrip boards as described before. This setup will help to increase the spatial resolution further.

在这个项目的框架内，我们希望开发一维核磁共振光谱成像技术，允许系统地研究3D细胞培养系统对抗癌药物的摄取。这些技术将允许在任何高分辨率核磁共振光谱仪上对质量和体积有限的样品进行空间分辨率和光谱分辨率高的核磁共振测量，而不需要任何专用硬件。在3D细胞培养系统中，代谢物和药物的分布可以用这项技术来确定，如球体，作为时间的函数。这项技术基于平面微带探测器，可以很容易地将微流控芯片作为样品载体来处理，以确保细胞的活力。该技术利用射频场和射频相位梯度进行成像，以避免机械干扰，并减少磁化率的不连续性对频谱的影响。为了产生射频场梯度，微带采用三角形结构，它产生电流密度的线性梯度和相应的射频场作为空间位移的函数。章动技术被用来获得空间分辨率。在此基础上，将研制一种具有射频相位梯度的探头，该探头包含如上所述的两个垂直排列的微带板。这种设置将有助于进一步提高空间分辨率。