Molecular characterization of transcription factors controlling indolic and aliphatic glucosinolate biosynthese in Arabidopsis thaliana

硫代葡萄糖苷是一类重要的植物次生代谢产物，在植物的生物胁迫反应中具有保护作用，是人类重要的抗癌物质。由于芥子油苷生物合成途径的转录控制以及芥子油苷在植物发育过程中的积累和对环境刺激的反应知之甚少，我们的目标是确定和表征拟南芥芥子油苷生物合成调控的新模式。为此，（i）将表征参与控制脂肪族（HAG 1和HAG 2）和吲哚芥子油苷（HIGH 1和HIGH 2）的生物合成途径的新转录因子;迄今为止功能未知的HAG 1/HAG 2和HIGH 1/HIGH 2属于R2 R3-MYB蛋白，其包含A.（ii）将鉴定构成脂肪族和吲哚硫代葡萄糖苷生物合成途径的相应靶基因，并阐明它们的共调节，（iii）将研究在植物发育期间和响应生物刺激时，HIGH 1/2和HAG 1/2对这些生物合成途径调节的影响，和（iv）一个假定的转运蛋白参与芥子油苷生物合成中间体的运输将被表征。

Glucosinolates belong to an important group of plant secondary metabolites that have protective functions in biotic stress response of plants and that are important anticancerogenic substances for humans. Because the transcriptional control of glucosinolate biosynthetic pathways as well as the accumulation of glucosinolates during plant development and in response to environmental stimuli are only poorly understood, we aim to identify and characterize new patterns of regulation of glucosinolate biosynthesis in Arabidopsis thaliana. To this end, (i) novel transcription factors involved in the control of biosynthetic pathways of aliphatic (HAG1 and HAG2) and indolic glucosinolates (HIG1 and HIG2) will be characterized; HAG1/HAG2 and HIG1/HIG2 with so far unknown functions belong to the R2R3-MYB proteins comprising one of the largest families of transcription factors in A. thaliana, (ii) the corresponding target genes constituting aliphatic and indolic glucosinolate biosynthetic pathways will be identified and their coregulation unraveled, (iii) the impact of HIG1/2 and HAG1/2 on the regulation of these biosynthetic pathways during plant development and in response to biotic stimuli will be studied, and (iv) a putative translocator involved in the transport of glucosinolate biosynthetic intermediates will be characterized.