Adenosine 3´,5´-bisphosphate (PAP)-mediated inter-compartmental metabolite signalling and intracellular transport of 3´-phosphoadenosine 5´-phosphosulfate (PAPS) and PAP in Arabidopsis thaliana

拟南芥中腺苷 3´,5´-二磷酸 (PAP) 介导的室间代谢信号传导以及 3´-磷酸腺苷 5´-磷酸硫酸盐 (PAPS) 和 PAP 的细胞内转运

• 批准号: 207585579
• 负责人: Professor Dr. Ulf-Ingo Flügge
• 金额: --
• 依托单位: Arbeitsgruppe Flügge (aufgelöst)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/207585579?language=en
• 关键词: Adenosine; bisphosphate; PAP; mediated; inter

Because plant cells are highly compartmentalized, they rely on an extensive exchange of signals between the different compartments to coordinate responses to a variety of internal and external cues. Recently, adenosine 3‘,5‘-bisphosphate (PAP) has been suggested to serve as a metabolic signal between plastids, mitochondria and nuclei. The phosphatase FRY1/SAL1 cleaving PAP into AMP and Pi is present in all three compartments. The increased accumulation of PAP in fry1/sal1 Arabidopsis thaliana knock-out plants causes changes in the expression of many nuclear-localized genes resulting in increased resistance to abiotic and biotic stresses such as drought, salt, high light or virus infection. It is aimed to get new insights on the role of FRY1/SAL1 and of plastidic (cPAPST1) and of mitochondrial (mPAPST1) PAP/PAPS transporters in intercompartmental PAP signalling. The role of each of these proteins in the generation, transduction and neutralization of the PAP signal in various cell compartments will be unravelled. To this end, (i) the transport characteristics of the organellar PAPS proteins will be assessed; (ii) genetic complementation of fry1 will be performed in which the expression of FRY1 is directed to only one of these compartments; (iii) the mutant plants containing different levels of PAP in either plastids, the cytosol or mitochondria will be generated and analyzed with respect to changes in plant appearance and resistances to abiotic stresses; (iv) the metabolite complementation of fry1/sal1 by crossing with mutants having manipulated levels of PAP in chloroplasts (apk1xapk2xapk4xfry1, apk1xapk2xapk4xcpapst1xfry1), mitochondria (mpapst1xfry1) or the cytosol (apk3xcpapst1xfry1, apk1xapk2xapk3xfry1, apk1xapk2xapk3xcpapst1xfry1) will be performed. These studies will include the determination of subcellular PAP levels.

由于植物细胞是高度区室化的，它们依赖于不同区室之间广泛的信号交换来协调对各种内部和外部线索的反应。最近，腺苷3'，5'-二磷酸（PAP）被认为是质体、线粒体和细胞核之间的代谢信号。磷酸酶FRY1/SAL1将PAP切割成AMP和Pi存在于所有三个隔室中。在fry1/sal1拟南芥基因敲除植物中PAP的积累增加导致许多核定位基因的表达变化，从而导致对非生物和生物胁迫如干旱、盐、强光或病毒感染的抗性增加。本研究旨在对FRY 1/SAL1、质体（cPAPST 1）和线粒体（mPAPST 1）PAP/PAPS转运蛋白在室间PAP信号传导中的作用有新的认识。这些蛋白质中的每一种在各种细胞隔室中PAP信号的产生、转导和中和中的作用将被阐明。为此，（i）将评估细胞器PAPS蛋白的转运特性;（ii）将进行fry1的遗传互补，其中FRY 1的表达仅针对这些区室之一;（iii）在任一质体中含有不同水平的PAP的突变体植物，产生细胞质或线粒体，并分析植物外观的变化和对非生物胁迫的抗性;（iv）通过与在叶绿体（apk1xapk2xapk4xfry1、apk1xapk2xapk4xcpapst1xfry1）、线粒体（mpapst1xfry1）或胞质溶胶（apk3xcpapst1xfry1、apk1xapk2xapk3xfry1、apk1xapk2xapk3xcpapst1xfry1）中具有操作水平的PAP的突变体杂交，进行fry1/sal 1的代谢物互补。这些研究将包括亚细胞PAP水平的测定。

项目成果

Sensing and signaling of oxidative stress in chloroplasts by inactivation of the SAL1 phosphoadenosine phosphatase

• DOI: 10.1073/pnas.1604936113
• 发表时间: 2016-08-02
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Chan, Kai Xun;Mabbitt, Peter D.;Pogson, Barry J.
• 通讯作者: Pogson, Barry J.