Maintenance of mitochondrial integrity in epidermal stem cells (A14*)

维持表皮干细胞线粒体完整性 (A14*)

• 批准号: 339973694
• 金额: --
• 依托单位: Institut für Vegetative Physiologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/339973694?language=en
• 关键词: Maintenance; mitochondrial; integrity; epidermal; stem

Progressive impairment of regeneration with age is partly due to a decline in tissue stem cell (SC) functionality. SCs have many mechanisms to minimize damage to preserve stemness and maintain their differentiation capacity, for which they also need intact mitochondria. The goal of this proposal is to test if mitochondrial DNA is protected against the accumulation of deletions in epidermal stem cells (EPSCs) by confining its replication rate to a minimum. For this purpose, we will genetically induce the generation of such deletions in EPSCs and test their functionality by various challenge strategies.

随着年龄的增长，再生的进行性损伤部分是由于组织干细胞（SC）功能的下降。SC具有许多机制来最小化损伤以保持干细胞性并维持其分化能力，为此它们还需要完整的线粒体。该提案的目标是测试是否通过将其复制率限制在最低限度来保护线粒体DNA免受表皮干细胞（EPSC）中缺失的积累。为此，我们将在EPSC中遗传诱导产生这种缺失，并通过各种挑战策略测试其功能。