120 keV Electron Microscope

120keV电子显微镜

• 批准号: 342247184
• 金额: --
• 依托单位: Universität Hamburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/342247184?language=en
• 关键词: 120; keV; Electron; Microscope

For an efficient structural characterisation in the outlined range of projects, samples need to be checked with respect to their quality and biochemical purity using electron microscopy of negative stained specimens, an easy and fast to perform contrasting method that allows reliable fast feedback. For this a 120keV, LaB6 transmission electron microscope is required. In the focus of the characterisation are evaluation of the sample monodispersity, structural integrity and homogeneity. A 120keV instrument equipped with latest electronics (hence reliable and long-term supported by the manufacturer), a LaB6 electron gun and versatile 4K CMOS detector fulfils these requirements. The instrument mediates the interface between (biochemical / biophysical) sample preparation/characterisation and the subsequent optimisation of vitrification etc. in the 200keV instrument before ultimate high-resolution data acquisition in the high-end 300 keV electron cryo microscopes. Moreover, the 120keV instrument requires capability of collection of initial datasets from negative stained samples, as first indicator and feedback for judging the success of a project before advancing to the cryo sample preparation stage. The instrument will not only be very versatile in its use but will furthermore crucially serve in the initial training of new users in electron (cryo) microscopy (e.g. introduction of vacuum system, electron optics, cryoEM initial training and data acquisition basics) without taking shares of valuable data collection time of the higher end cryoEMs.

为了在概述的项目范围内进行有效的结构表征，需要使用负染色标本的电子显微镜检查样品的质量和生化纯度，这是一种简单快速的对比方法，可以提供可靠的快速反馈。为此，需要120 keV的LaB 6透射电子显微镜。表征的重点是评价样品的单分散性、结构完整性和均匀性。配备最新电子设备的120 keV仪器（因此可靠且得到制造商的长期支持），LaB 6电子枪和多功能4K CMOS检测器满足这些要求。该仪器在高端300 keV电子低温显微镜中进行最终高分辨率数据采集之前，在200 keV仪器中调解（生物化学/生物物理）样品制备/表征与后续玻璃化优化等之间的接口。此外，120 keV仪器需要能够从阴性染色样品中收集初始数据集，作为在进入冷冻样品制备阶段之前判断项目成功的第一指标和反馈。该仪器不仅用途广泛，而且还将在电子（低温）显微镜新用户的初始培训中发挥重要作用（例如真空系统介绍，电子光学，cryoEM初始培训和数据采集基础知识），而无需分享高端cryoEM的宝贵数据收集时间。