Zur Evolution der Larvenformen der Bivalvia (Mollusca): Ein high-tech-mikromorphologischer Vergleich

双壳纲（软体动物）幼虫形态的进化：高科技微形态比较

• 批准号: 34384108
• 负责人: Professor Dr. Gerhard Haszprunar
• 金额: --
• 依托单位: Zoologische Staatssammlung München
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/34384108?language=en
• 关键词: Zur; Evolution; der; Larvenformen; Bivalvia

The comparative-ontogenetic data on various molluscan groups, which have been generated by application of a broad methodology within the foregoing projects, should be widened concerning the protobranch Bivalvia. The remaining main topic is the clarification of the status of the pericalymma larva of the Protobranchia. Representatives of the respective taxa (mainly Nuculidae) will be collected alive and their larva will be bred. The various stages will be investigated by several high-tech morphological (light- and electron microscopy, computer-aided 3D-reconstruction, fluorescence labeling) methods. Objectives are: (1) Show the myo- und (2) in parts also neurogenesis of the different larval types by fluorescent dyes coupled with Confocal-Laser- Scanning-Microscopy (CLSM). Provide SEM/TEM-data on the (3) prototroch and on larval (4) sensory and (5) excretory organs and compare them with homologous structures in other molluscan classes. (6) Summarize the data to provide an evolutionary phylogeny (evo-devo) of Bivalvia and beyond. The main objectives have direct consequences for the bivalvian phylogeny and for a new framework about the origin and evolutionary history of the phylum Mollusca.

在上述项目中应用广泛的方法所产生的各种软体动物类群的比较个体发生数据，应扩大到双壳纲的原分支。剩下的主要议题是澄清原鳃的周膜幼虫的地位。将活捉各分类群的代表（主要是nucululdae），并繁殖其幼虫。各个阶段将通过几种高科技形态学（光学和电子显微镜，计算机辅助3d重建，荧光标记）方法进行调查。目的：(1)利用荧光染料结合共聚焦激光扫描显微镜（CLSM）观察不同类型幼虫的肌肉和部分神经发生。提供(3)原软体动物和(4)幼虫感觉器官和(5)排泄器官的SEM/ tem数据，并将其与其他软体动物类的同源结构进行比较。(6)总结资料，提供Bivalvia及以后的进化系统发育（evo-devo）。主要目标对双壳动物系统发育和软体动物门起源和进化历史的新框架有直接影响。