权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

腸管膜腸液・粘液分泌が関わる排便機構の解明：遺伝要因を考慮した便秘の分類と改善

阐明涉及肠液和粘液分泌的排便机制：考虑遗传因素的便秘分类和改善

基本信息

批准号：
19K11804
负责人：
浦本裕美
金额：
$ 2.75万
依托单位：
Jin-ai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2019
资助国家：
日本
起止时间：
2019-04-01 至 2024-03-31
项目状态：
已结题

项目摘要

ヒトでの研究が困難になったため、上記テーマの解明に向けて動物実験によりCFTR機能低下便秘モデルマウス作成の検討を行った。即ち、腸管膜に発現しているCFTRの機能を阻害することで便秘を誘導できるのかを、CFTR特異的阻害剤であるCFTRinh172をマウスに経口投与し確認した。阻害剤投与群の1日当たりの平均摂薬量は24.68μgであった。阻害剤投与群の体重変化と解剖時の肝臓重量をコントロール群と較べると違いはなく、本実験での投与量および投与期間では、CFTR阻害剤の摂取は、マウスの成長や健康に影響を与えなかったと考えられた。乾燥糞量は阻害剤投与の影響はなく、ペレット数は阻害剤投与により数は減ったが、有意差は認められなかった。糞水分量は、阻害剤投与1週間経過後の約1週間の観察で漸減傾向を示し、また、コントロール食に切替えるとすみやかに水分量は増え、回復期間5日目でコントロールマウスの結果とほぼ同じレベルであることから、5日目で回復は平衡状態に達していたと考えられた。阻害剤投与13日目と15日目は回復期5日目に較べ糞水分率は少なく、13日目で有意に少なかった（p<0.05）。阻害剤投与の腸内糞通過時間への影響を阻害剤投与8日目で観察した結果、コントロール群では色素投与から完全に糞の着色が無くなるまでに約24時間かかったのに対し、阻害剤投与群では26～30時間かかっており、腸内糞通過時間の遅延が確認できた。コントロール食に切替えたタイミングでの結果は、コントロール群と阻害剤投与群で差はなく、阻害剤投与を止めることですみやかにコントロール群と同等にまで回復することがわかった。以上から、マウスにおいて、CFTR阻害剤を毎日40μg（正味摂取量約20μg/日）を餌に加え約2週間投与することで便秘状態を誘導できると考えられた。

In the study, you need to know that the CFTR machine can lower the constipation and make the constipation machine into a constipation machine. That is to say, the tube membrane shows that the CFTR machine can prevent the constipation from causing the constipation, and the CFTR can prevent the constipation from the constipation, and the CFTR can prevent the constipation from getting rid of the pain. On the 1st, the average dose is 24.68 μ g. During autopsy, the liver weight test was performed during dissection, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, the liver weight test was performed during autopsy, and The amount of dryness, the amount of damage, the amount of damage. After the loss of water, the loss of water and the amount of water, it is necessary to monitor the amount of water in the water supply for about 1 minute after the operation, and to monitor the amount of water in the water supply during the return period. during the return period, the results show that the results are the same as those in the same period, and the balance is in good condition. On the 13th, the water content of the 15th eye was lower than that of the 13th, and the moisture content of the 13th eye was intentionally lower than that of the 13th eye (0.05). The results of the 8-day visual inspection of the results In the first place, the results, the results and the results were analyzed. The above antiseptic drugs and CFTR prevent 40 μ g (about 20 μ g