Cold atmospheric plasmas for the study of fundamental interaction mechanisms with biological substrates: the role of ions and the plasma-induced liquid chemistry

等离子体与生物底物相互作用的兴趣正在迅速增长，在食品、制药和医疗行业具有巨大的应用潜力，包括各种治疗应用。血浆去污本身是快速的，不留下有毒化合物的痕迹，并且可以处理热敏底物，包括活组织。我们的提案包PAK728等离子体-微生物相互作用在等离子体表面去污（PlasmaDecon）期间，通过研究活性物质与细菌和孢子之间的基本相互作用机制以及活性物质、光子和热量之间的协同效应，对理解等离子体去污过程中不同应用的基本过程做出了重大贡献。我们还能够提供等离子体对生物大分子的影响的分子水平的见解。这一更新提案旨在回答在PlasmaDecon的研究过程中出现的新问题，以及其他研究人员在该领域提出的新问题，主要关注两个新主题：1)研究反应物质从等离子体到液体的运输，然后研究液相中产生的化学反应。这些数据将作为表征等离子体活化水对生物基质的影响的起点。2)使用VUV辐射（来自等离子体和商用灯），以这种方式产生绝对校准的光电离产生的离子通量到基底。这些离子诱导细胞和细胞结构损伤的分子机制将被研究。拟议的研究将为其他研究人员提供重要的，因此有吸引力的结果，它已经吸引了五个合作伙伴：教授。RUB的J. Bandow、K. Stapelmann和P. Awakowicz（来自PAK728的合作者）以及peter Lukes博士（捷克共和国布拉格）和Felipe Iza博士（英国拉夫堡）。

The interest in plasma interactions with biological substrates is rapidly growing, with large application potential in food, pharmaceutical, and medical industries including a variety of therapeutic applications. Plasma decontamination is inherently fast, do not leave traces of toxic compounds, and can treat heat-sensitive substrates including living tissues. Our proposal package PAK728 Plasma-Microbe Interaction during Plasma-based Surface Decontamination (PlasmaDecon) has contributed significantly to the understanding of the fundamental processes underlying the different applications of plasma decontamination processes by studying the fundamental interaction mechanisms between reactive species and bacteria and spores and also by studying synergistic effects between reactive species, photons, and heat. We were also able to provide molecular-level insights into the effects of plasma on bio-macromolecules. This renewal proposal aims at answering new questions, which have surfaced during the investigations in PlasmaDecon and with have been raised by other researchers in the field and focuses mainly on two new topics: 1) Investigation of the transport of reactive species from the plasma into a liquid, followed by the study of the resulting chemistry in the liquid phase. These data will serve as a starting point in the characterization of the effects of the plasma activated water on the biological substrates. 2) Use of VUV radiation (from plasma and commercial lamps) in such a way that an absolutely calibrated flux of photoionization-generated ions to the substrate is generated. The molecular mechanisms of damage of cells and cellular structures induced by these ions will be investigated. The proposed research will provide important and therefore attractive results for other researchers and it already attracted five collaboration partners: Profs. J. Bandow, K. Stapelmann, and P. Awakowicz at RUB (collaborators from the PAK728) and Dr. Petr Lukes (Prague, Czech Republic) and Dr. Felipe Iza (Loughborough, United Kingdom).