Cold atmospheric plasmas for the study of fundamental interaction mechanisms with biological substrates: the role of ions and the plasma-induced liquid chemistry

用于研究与生物基质的基本相互作用机制的冷大气等离子体：离子的作用和等离子体诱导的液体化学

• 批准号: 356768018
• 负责人: Professor Jan Benedikt, Ph.D.
• 金额: --
• 依托单位: Institut für Experimentelle und Angewandte Physik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/356768018?language=en
• 关键词: Cold; atmospheric; plasmas; study; fundamental

The interest in plasma interactions with biological substrates is rapidly growing, with large application potential in food, pharmaceutical, and medical industries including a variety of therapeutic applications. Plasma decontamination is inherently fast, do not leave traces of toxic compounds, and can treat heat-sensitive substrates including living tissues. Our proposal package PAK728 Plasma-Microbe Interaction during Plasma-based Surface Decontamination (PlasmaDecon) has contributed significantly to the understanding of the fundamental processes underlying the different applications of plasma decontamination processes by studying the fundamental interaction mechanisms between reactive species and bacteria and spores and also by studying synergistic effects between reactive species, photons, and heat. We were also able to provide molecular-level insights into the effects of plasma on bio-macromolecules. This renewal proposal aims at answering new questions, which have surfaced during the investigations in PlasmaDecon and with have been raised by other researchers in the field and focuses mainly on two new topics: 1) Investigation of the transport of reactive species from the plasma into a liquid, followed by the study of the resulting chemistry in the liquid phase. These data will serve as a starting point in the characterization of the effects of the plasma activated water on the biological substrates. 2) Use of VUV radiation (from plasma and commercial lamps) in such a way that an absolutely calibrated flux of photoionization-generated ions to the substrate is generated. The molecular mechanisms of damage of cells and cellular structures induced by these ions will be investigated. The proposed research will provide important and therefore attractive results for other researchers and it already attracted five collaboration partners: Profs. J. Bandow, K. Stapelmann, and P. Awakowicz at RUB (collaborators from the PAK728) and Dr. Petr Lukes (Prague, Czech Republic) and Dr. Felipe Iza (Loughborough, United Kingdom).

对等离子体与生物基质相互作用的兴趣正在迅速增长，在食品、制药和医疗行业中具有巨大的应用潜力，包括各种治疗应用。等离子体去污本身快速，不会留下有毒化合物的痕迹，并且可以处理包括活组织在内的热敏基质。我们的提案包PAK 728等离子体-微生物相互作用等离子体表面去污（PlasmaDecon）通过研究反应性物种与细菌和孢子之间的基本相互作用机制，以及研究反应性物种，光子和热量之间的协同效应，为理解等离子体去污过程的不同应用的基本过程做出了重大贡献。我们还能够提供分子水平的见解，血浆对生物大分子的影响。该更新提案旨在回答在PlasmaDecon研究期间出现的新问题，以及该领域其他研究人员提出的新问题，主要集中在两个新主题上：1）研究活性物质从等离子体到液体的运输，然后研究液相中产生的化学反应。这些数据将作为等离子体活化水对生物基质的影响的表征的起点。2)使用真空紫外辐射（来自等离子体和商业灯），以产生绝对校准的光电离产生的离子流到基板。将研究这些离子引起细胞和细胞结构损伤的分子机制。拟议的研究将为其他研究人员提供重要且具有吸引力的结果，并且已经吸引了五个合作伙伴：教授。J. Bandow，K. Stapelmann，and P. Awakowicz at RUB（collaborators from the PAK 728）and Dr. Petr Lukes（布拉格，捷克共和国）and Dr. Felipe Iza（拉夫堡，英国）.