Protein-protein interaction in the mammalian circadian system

哺乳动物昼夜节律系统中蛋白质-蛋白质相互作用

• 批准号: 35756735
• 负责人: Professor Dr. Achim Kramer
• 金额: --
• 依托单位: Fachbereich Chronobiologie (CVK)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/35756735?language=en
• 关键词: Protein; protein; interaction; mammalian; circadian

Circadian clocks are endogenous oscillators that regulate daily rhythms in physiology and behaviour. In almost every cell a gene-regulatory network generates molecular circadian oscillations with protein phosphorylation and complex formation playing a crucial role for their dynamics. Here, we will analyze these interactions (with PER2 as paradigmatic protein) both on the molecular and the functional level. To this end, we aim to identify kinases phosphorylating PER2, characterize its interactions with CRY1 and CKIε/δ as well as pharmacologically perturb these interactions in an established dynamical cell-culture model with subsequent analysis of the effect on circadian rhythms.

生物钟是调节日常生理和行为节奏的内源性振荡器。在几乎每个细胞中，基因调控网络产生分子昼夜节律振荡，蛋白质磷酸化和复合物形成在其动力学中起着至关重要的作用。在这里，我们将在分子和功能水平上分析这些相互作用（以PER2为范例蛋白）。为此，我们的目标是鉴定磷酸化PER2的激酶，表征其与CRY1和CKIε/δ的相互作用，以及在建立的动态细胞培养模型中从药理学上干扰这些相互作用，随后分析对昼夜节律的影响。