Development of an interface for the on-line coupling of HPLC and HR-cs-GFAAS for fluorine speciation analysis

开发用于氟形态分析的 HPLC 和 HR-cs-GFAAS 在线耦合接口

基本信息

项目摘要

In the field of pharmaceuticals fluorination of organic molecules leads to new, promising properties (e.g. changed metabolism of drugs). Fluorinated substances are also beneficial in the area of technical applications.Growing production volumes and application fields lead to an increase of these substances within the (aquatic) environment. Due to their new properties a different distribution, speciation as well as bioavailability in the environment is most likely.Analytical techniques comprising high sensitivity, fluorine-selectivity as well as a fluorine species-unspecific detector response are missing. Thus, the main objective of this project is the development of a complementary method, which enables fluorine-speciation analysis for various applications including analysis of (aquatic) environmental samples to assess the load situation, distribution and bioavailability as well as speciation of fluorinated compounds. Further fields that would profit from such method are pharmaceutical, medical as well as material sciences.High resolution-continuum source -graphite furnace atomic absorption spectrometry (HR-cs-GFAAS) enables fluorine detection in the molecular absorption (MAS) mode. However, up to now HR-cs-GFAAS has not been coupled on-line with a separation systems (e.g. HPLC), which is mandatory for fluorine speciation analysis.Thus, the main objectives of this project are:I) Coupling-interface designA new interface comprising a micro-droplet generator for the on-line coupling of nanoHPLC with HR-cs-GFMAS will be designed, constructed and installed. In addition the adaption and optimization of the graphite furnace heating program for transient signals as well as raw data extraction and data evaluation will be tackled.II) Fluorine SpeciationThe parameters of the newly developed interface for fluorine speciation analysis will be optimized with fluorinated test substances. Furthermore, a nanoHPLC separation method for several fluorine-containing organic test substances will be developed to investigate the species-unspecific response of GFMAS in dependence of organic solvent gradients. The developed system will be validated in terms of LOD, LOQ, linearity, robustness, intra- & inter-day reproducibility.III) Real sample analysisWater sample preparation as well as sample/species enrichment strategies for fluorinated organic substances and related species with regard to expected low environmental concentrations will be developed and optimized. Water samples from waste water treatment plants as well as surface waters will be sampled and analyzed by means of nanoHPLC/HR-cs-GFMAS and complementary nanoHPLC/ESI-Q-ToF-MS to unravel molecular structures of the detected fluorine species.IV) Suitability for routine analysisSimplification of the newly developed interface is planned to obtain an applicable method for fluorine speciation which is transferable to other laboratories as well as further fields of application.
在制药领域,有机分子的改性导致新的、有希望的性质(例如改变药物的代谢)。含氟物质在技术应用领域也是有益的,生产量和应用领域的增长导致这些物质在(水生)环境中的增加。由于它们的新特性,环境中最有可能出现不同的分布、形态和生物利用度。缺乏包括高灵敏度、氟选择性以及氟物种非特异性检测器响应的分析技术。因此,该项目的主要目标是开发一种补充方法,使氟形态分析能够用于各种应用,包括分析(水生)环境样本,以评估氟化合物的负载情况、分布和生物利用度以及形态。高分辨率连续光源石墨炉原子吸收光谱法(HR-cs-GFAAS)可以在分子吸收(MAS)模式下进行氟的检测。然而,到目前为止,HR-cs-GFAAS还没有与分离系统(例如HPLC)在线耦合,这是氟形态分析所必需的。因此,本项目的主要目标是:I)耦合接口设计一个新的接口,包括一个微滴发生器,用于nanoHPLC与HR-cs-GFMAS的在线耦合。此外,将解决的适应和优化的石墨炉加热程序的瞬态信号,以及原始数据提取和数据评估。II)氟形态新开发的接口的参数氟形态分析将优化与氟化测试物质。此外,将开发几种含氟有机测试物质的nanoHPLC分离方法,以研究GFMAS依赖于有机溶剂梯度的物种非特异性响应。所开发的系统将在LOD,LOQ,线性,鲁棒性,内&日间重现性方面进行验证。III)真实的样品分析将开发和优化水样制备以及针对预期低环境浓度的氟化有机物质和相关物种的样品/物种富集策略。将对废水处理厂的水样以及地表沃茨进行采样,并通过nanoHPLC/HR-cs-GFMAS和补充nanoHPLC/ESI-Q-ToF-MS进行分析,以揭示检测到的氟物质的分子结构。IV)计划对新开发的界面进行简化,以获得一种适用于氟形态分析的方法,该方法可转移到其他实验室以及其他领域,应用程序.

项目成果

期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Trendbericht Analytische Chemie 2022
  • DOI:
    10.1002/nadc.20224123794
  • 发表时间:
    2022-05
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Fabian Simon;Lennart Gehrenkemper;Marcus von der Au;Heike Traub;J. Vogl;Björn Meermann;Georg Steinhaus
  • 通讯作者:
    Fabian Simon;Lennart Gehrenkemper;Marcus von der Au;Heike Traub;J. Vogl;Björn Meermann;Georg Steinhaus
A fast and simple PFAS extraction method utilizing HR-CS-GFMAS for soil samples.
  • DOI:
    10.1016/j.chemosphere.2022.133922
  • 发表时间:
    2022-02
  • 期刊:
  • 影响因子:
    8.8
  • 作者:
    Fabian Simon;Lennart Gehrenkemper;M. Au;Philipp Wittwer;P. Roesch;Jens Pfeifer;Antje Cossmer;
  • 通讯作者:
    Fabian Simon;Lennart Gehrenkemper;M. Au;Philipp Wittwer;P. Roesch;Jens Pfeifer;Antje Cossmer;
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Dr. Björn Meermann其他文献

Dr. Björn Meermann的其他文献

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{{ truncateString('Dr. Björn Meermann', 18)}}的其他基金

Development of analytical methods for speciation analysis of copper and zinc-based antifouling agents in surface water
地表水中铜基和锌基防污剂形态分析方法的开发
  • 批准号:
    321800101
  • 财政年份:
    2016
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Development of an Automated On-line Hyphenation of Capillary Electrophoresis with Multicollector ICP-MS for Species-specific Isotopic Analysis of Biomolecules
开发毛细管电泳与多接收器 ICP-MS 的自动在线联用,用于生物分子的物种特异性同位素分析
  • 批准号:
    440953647
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
    Research Grants

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