Bildgebendes Massenspektrometer

成像质谱仪

• 批准号: 361026751
• 金额: --
• 依托单位: Heinrich-Heine-Universität Düsseldorf
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/361026751?language=en
• 关键词: Bildgebendes; Massenspektrometer

For the elucidation of the mechanism of biological processes the molecular characterization of tissue samples is of great interest. Besides a qualitative and quantitative description of involved molecules the knowledge about spatial distribution of these biomolecules is essential for our understanding of complex cellular organisation and dynamics. One technique that has emerged for this purpose is imaging mass spectrometry as a label-free technique without prior knowledge of the analyte. The Heinrich-Heine University of Düsseldorf (HHU) does not possess a mass spectrometer with imaging capabilities. The use of a mass spectrometer for a high-resolution (5 μm) MALDI-imaging with MS/MS capabilities for the detailed characterization of the analyte is required for the following projects at the HHU: Investigation of the fine structure of the plant cell walls of various cells and parts thereof by cell-specific oligosaccharide mass profiling (COLIMP) and the use of this technique to investigate a plethora of cell wall mutants and specialized cells e.g. those involved in C4 photosynthesis. The cell specific determination of specific C4 protein complexes by their peptide profiles and lipid and sterol profiles. The dynamics and posttranslational modifications of stem cell signalling molecules such as CLE.

为了阐明生物过程的机制，组织样品的分子表征是非常有意义的。除了对相关分子的定性和定量描述外，关于这些生物分子的空间分布的知识对于我们理解复杂的细胞组织和动力学是必不可少的。为此出现的一种技术是成像质谱学，它是一种无需事先了解分析物的无标记技术。杜塞尔多夫海因里希-海涅大学(HHU)没有具有成像能力的质谱仪。HHU的下列项目需要使用具有MS/MS能力的高分辨率(5μm)MALDI成像的质谱仪对分析物进行详细表征：通过细胞特异性寡糖质量图谱研究不同细胞及其部分的植物细胞壁的精细结构，并利用这项技术研究过多的细胞壁突变和特殊细胞，如参与C4光合作用的细胞。细胞特异性测定特定的C4蛋白复合体，通过它们的多肽谱、脂类和甾醇谱。干细胞信号分子的动力学和翻译后修饰，如CLE。