Bildgebendes Massenspektrometer

为了阐明生物过程的机制，组织样品的分子表征具有很大的意义。除了对所涉及的分子进行定性和定量描述外，关于这些生物分子的空间分布的知识对于我们理解复杂的细胞组织和动力学是必不可少的。为此目的出现的一种技术是成像质谱法，作为一种无标签技术，无需事先了解被分析物。德国<s:1>塞尔多夫海因里希-海涅大学（HHU）没有具备成像能力的质谱仪。使用一个高分辨率质谱仪(5μm) MALDI-imaging MS / MS功能的详细描述分析物需要以下项目在HHU:调查植物细胞壁的精细结构的各种细胞和部分特异性寡糖质量分析(COLIMP)和使用这种技术来调查大量的细胞壁突变体和专门的细胞如参与C4光合作用。细胞特异性测定特定C4蛋白复合物的肽谱和脂质和固醇谱。干细胞信号分子如CLE的动态和翻译后修饰。

For the elucidation of the mechanism of biological processes the molecular characterization of tissue samples is of great interest. Besides a qualitative and quantitative description of involved molecules the knowledge about spatial distribution of these biomolecules is essential for our understanding of complex cellular organisation and dynamics. One technique that has emerged for this purpose is imaging mass spectrometry as a label-free technique without prior knowledge of the analyte. The Heinrich-Heine University of Düsseldorf (HHU) does not possess a mass spectrometer with imaging capabilities. The use of a mass spectrometer for a high-resolution (5 μm) MALDI-imaging with MS/MS capabilities for the detailed characterization of the analyte is required for the following projects at the HHU: Investigation of the fine structure of the plant cell walls of various cells and parts thereof by cell-specific oligosaccharide mass profiling (COLIMP) and the use of this technique to investigate a plethora of cell wall mutants and specialized cells e.g. those involved in C4 photosynthesis. The cell specific determination of specific C4 protein complexes by their peptide profiles and lipid and sterol profiles. The dynamics and posttranslational modifications of stem cell signalling molecules such as CLE.