Analysis of pattern formation in the epidermis of the adult abdomen of Drosophila by in vivo 4D-microscopy

体内 4D 显微镜分析果蝇成体腹部表皮的图案形成

• 批准号: 36125941
• 负责人: Dr. Marcus Bischoff
• 金额: --
• 依托单位: Department of Zoology
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/36125941?language=en
• 关键词: Analysis; pattern; formation; epidermis; adult

How are complex patterns formed by groups of cells? Different cellular processes have to work together dynamically to position and orient all cells to ensure proper development. I aim to investigate pattern formation in the adult abdominal epidermis of Drosophila by in vivo 4D-microscopy. The abdominal epidermis of Drosophila is well suited to study pattern formation: it is formed anew during metamorphosis by a variety of different processes, including cell sorting and cell movements plus the establishment of a strict antero-posterior polarity in the epithelium. 4D-microscopy allows the analysis of cell behaviour at the level of single cells by generating movies that record a process in both space and time. I will combine this imaging technique with clonal analysis to study the effects of removing or over-expressing certain gene products on cell behaviour. I will focus on genes which affect cellular polarity and identity and are known to be important for patterning. This will allow me to gain insights into the mechanisms underlying cell movements, cell shape changes, and cellular polarity and to find new aspects of cell behaviour during the extensive cell movements and cell divisions that occur while the adult abdomen is being built.

细胞群是如何形成复杂图案的？不同的细胞过程必须动态地协同工作，以定位和定向所有细胞，以确保正确的发育。我的目的是调查图案形成在果蝇的成年腹部表皮在体内的4D显微镜。果蝇的腹部表皮非常适合研究图案的形成：它在变态期间通过各种不同的过程重新形成，包括细胞分选和细胞运动以及在上皮中建立严格的前后极性。4D显微镜通过生成记录空间和时间过程的电影，允许在单细胞水平上分析细胞行为。我将联合收割机这种成像技术与克隆分析相结合，以研究去除或过度表达某些基因产物对细胞行为的影响。我将集中讨论影响细胞极性和身份的基因，这些基因对模式化很重要。这将使我能够深入了解细胞运动、细胞形状变化和细胞极性的机制，并在成人腹部发育过程中发生的广泛细胞运动和细胞分裂过程中发现细胞行为的新方面。