The genomic architecture of convergent snail crushing jaws in cichlid fishes

丽鱼科鱼类会聚蜗牛粉碎颚的基因组结构

• 批准号: 366312182
• 负责人: Professor Dr. C. Darrin Hulsey
• 金额: --
• 依托单位: School of Biology and Environmental Science
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/366312182?language=en
• 关键词: genomic; architecture; convergent; snail; crushing

Convergent phenotypes that arise during adaptive radiation could often result from similar changes in genomic architecture. The goal of this proposal is to test hypotheses regarding the genomic architecture of convergence in crushing pharyngeal morphology in several lineages of cichlid fish. The outlined approach integrates mapping of the position of genomic regions affecting traits such as tooth size, muscle mass, and jaw suturing within the genome. The proposal addresses three major questions:Objective 1: What genomic region(s) are associated with the polymorphism in tooth size found in H. minckleyi? Using a hybrid F2 mapping cross, we will determine if there is a single locus of large effect or alternatively multiple regions of the genome associated with bimodal size of the pharyngeal teeth in H. minckleyi. Objective 2: Do the QTL for other pharyngeal jaw traits associated with the tooth size polymorphism in wild-caught H. minckleyi co-localize to tooth size QTL? We will use the QTL identified in Objective 1 to determine if QTL identified for other pharyngeal jaw traits associated with the molariform versus papilliform tooth size dichotomy co-localize to homologous regions of the genome where tooth size QTL(s) are present. We will also evaluate whether the genomic architecture for these additional traits themselves are exceptionally co-localized to similar regions of the genome.Objective 3: Is the genomic architecture associated with the crushing phenotypes in other cichlid lineages similar to that found in H. minckleyi? We will use four additional hybrid crosses of species that differ along a molariform to papilliform axis to determine if species pairs that span cichlid diversity exhibit the same number of QTLs, same degree of co-localization, as well as QTL in the homologous genomic locations that we recovered for the pharyngeal jaw divergence in polymorphic populations of H. minckleyi.The QTL studies performed in a number of lineages that have diverged along a similar phenotypic axis should provide a test of whether similar or very distinct genomic loci have generated pharyngeal jaw convergence within populations and across species of cichlid fishes.

在适应性辐射过程中出现的趋同表型通常可能是由基因组结构的类似变化引起的。这项建议的目的是测试假设的基因组架构的收敛在破碎咽形态在几个谱系的慈鲷鱼。概述的方法集成了影响性状的基因组区域的位置的映射，如牙齿大小，肌肉质量和基因组内的颌骨。该提案解决了三个主要问题：目标1：什么基因组区域与在H中发现的牙齿大小多态性相关。minckleyi？利用杂种F2作图杂交，我们将确定是否有一个单一的基因座的大的影响，或者替代的多个区域的基因组与双峰大小的咽齿在H。minckleyi。目的2：对野生哈氏哈minckleyi共定位到牙齿大小QTL？我们将使用在目标1中鉴定的QTL来确定与臼齿状与乳头状牙齿大小二分法相关的其他咽颌性状鉴定的QTL是否共定位于存在牙齿大小QTL的基因组的同源区域。我们还将评估这些额外性状本身的基因组结构是否异常地共定位于genome.Objective 3的相似区域：与其他慈鲷科谱系中的压碎表型相关的基因组结构是否与在H. minckleyi？我们将使用四个额外的杂交品种，不同沿着臼齿状到乳突状轴，以确定是否物种对跨越慈鲷多样性表现出相同数量的QTL，相同程度的共定位，以及QTL的同源基因组位置，我们恢复了多态性群体的H。在沿着沿着相似的表型轴分化的许多谱系中进行的QTL研究应该提供相似或非常不同的基因组基因座是否在群体内和在慈鲷属鱼类的物种之间产生咽颌会聚的测试。