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Four-dimensional prediction and quantification of how physical forces impact organogenesis in zebrafish

物理力如何影响斑马鱼器官发生的四维预测和量化

基本信息

批准号：
10271304
负责人：
JEFFREY D AMACK
金额：
$ 45.35万
依托单位：
SYRACUSE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-25 至 2025-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10271304
关键词：
3-Dimensional Ablation Actomyosin Address Affect Anterior Architecture Biochemical Biological Models Biomechanics Biophysical Process Biophysics Cell Shape Cell model Cells Cellular biology Complement Complex Congenital Abnormality Defect Developmental Biology Developmental Process Dorsal Embryo Embryonic Development Environment Epithelial Extracellular Matrix Formulation Foundations Four-dimensional Goals Health Image Image Analysis Individual Knowledge Lasers Lead Left Mathematics Measurement Mechanics Methods Mission Modeling Morphogenesis Motion Movement Optics Organ Organ Model Organogenesis Output Pattern Phenotype Physics Play Prevention Public Health Research Resolution Rod Role Shapes Signal Transduction Signaling Molecule Structural Congenital Anomalies Structure Surface Testing Three-dimensional analysis Time Tissues United States National Institutes of Health Velocimetries Vesicle Work Zebrafish base cell motility convergent extension disability in vivo imaging malformation mathematical model mechanical force mechanical properties morphogens multidisciplinary notochord novel particle predictive modeling prevent programs simulation three dimensional structure

项目摘要

PROJECT SUMMARY/ABSTRACT Defects in programmed cell shape changes during embryonic development can disrupt organ morphogenesis and cause structural birth defects. There are fundamental gaps in our understanding of how cells change their shape during organ formation. While the biochemical signals and morphogen gradients that help govern organogenesis are well-studied, evidence is growing that robust control of organ form and function often also depends on multiple mechanical mechanisms that remain poorly understood. Thus, there is a critical need to tease apart how multiple mechanisms – including tissue-scale dynamic forces and cell-autonomous contractile forces – work together to generate “mechanical gradients” that program cell and organ shape during organ formation. A challenge is that mechanical perturbations that affect the entire embryo often result in the same global phenotype, making it difficult to pinpoint the role of each mechanism. Our long- term goal is to develop a combined cell biology and modeling toolkit that allows us to predict cell-scale phenotypes and appropriate perturbations that can be used to distinguish between multiple mechanical mechanisms. This project uses Kupffer’s vesicle (KV), a transient epithelial organ that establishes left-right asymmetry in the zebrafish embryo, as a model system. No upstream biochemical signaling gradients have been identified that regulate KV cell shapes as required for left-right patterning, but multiple mechanical mechanisms have been implicated. Preliminary results – from (4D = 3D + time) experimental perturbations and measurements of single KV cell shapes, and novel mathematical models that simulate interacting 3D tissue structures while retaining cell-scale resolution – lead us to formulate our central hypothesis that cell shape changes critical for KV organogenesis result from mechanical gradients generated by interactions between the KV and surrounding tissue structures as well as cell-autonomous contractile forces from inside KV. The goal of Aim 1 is to determine how interactions between KV and notochord impact cell shape changes. 4D modeling predictions for cell shapes and cell movement combined with live in vivo imaging and localized laser ablations will determine how asymmetric forces generated by the rod-like notochord impact KV cell shape changes during organogenesis. The goal of Aim 2 is to understand mechanisms by which actomyosin contractility in surrounding tailbud cells and inside KV generate KV cell shape changes. Novel mathematical models will predict how localized optical perturbations to tailbud mechanics, as well as perturbations to volume and cell- autonomous contractility in cells inside the KV, affect KV organ shape. Key outputs include a modeling toolkit for high-throughput simulations of dynamic interactions between complex 3D tissue structures complemented by a cell biology toolkit that tests model predictions with spatially and temporally modulated activation of biomechanical and biochemical signaling molecules. These results will pinpoint mechanical mechanisms that regulate organogenesis, and may ultimately aid in the prediction or prevention of birth defects.

项目总结/文摘