Characterization of Blumeria graminis peptides as potential effectors targeting barley RAC/ROP proteins

小麦白粉菌肽作为针对大麦 RAC/ROP 蛋白的潜在效应物的表征

• 批准号: 178859977
• 负责人: Professor Dr. Ralph Hückelhoven
• 金额: --
• 依托单位: Lehrstuhl für Phytopathologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/178859977?language=en
• 关键词: Characterization; Blumeria; graminis; peptides; potential

Small monomeric RAC/ROP GTPases are molecular switches in cell polarity and plant defence responses. In barley RACB and related RAC/ROPs function in susceptibility to the biotrophic ascomycete pathogen Blumeria graminis f.sp. hordei (Bgh). To identify RACBinteracting proteins, we have carried out yeast two hybrid screenings of a cDNA library from infected barley. This identified six fungal clones coding for to the same class of small peptides. Protein-protein interaction was confirmed in independent assays, and a GFP-fusion protein of a fungal RACB-interacting peptide labelled small structures in the cytoplasm and cell cortex and accumulated at the site of pathogen entry. Over-expression or host-induced gene silencing of two Bgh-peptides, enhanced or reduced susceptibility to fungal entry, respectively. By the use of cell-biological and genetic tools, we would like now to provide evidence that the peptides represent fungal virulence effectors that target barley RAC/ROPs for effector-triggered susceptibility.

小单体RAC/ROP GTP酶是细胞极性和植物防御反应的分子开关。在大麦中，RACB和相关RAC/ROP在对生养型子囊菌病原体大麦白粉病菌（Blumeria graminis f.sp. hordei，Bgh）的易感性中起作用。为了鉴定与RACB相互作用的蛋白质，我们利用酵母双杂交技术对感染的大麦cDNA文库进行了筛选。这鉴定了编码同一类小肽的六个真菌克隆。蛋白质-蛋白质相互作用在独立的测定中得到证实，并且真菌RACB相互作用肽的GFP融合蛋白标记了细胞质和细胞皮质中的小结构，并在病原体进入的位点处积累。过表达或宿主诱导的基因沉默的两个BGH-肽，增强或降低的易感性真菌进入，分别。通过使用细胞生物学和遗传学工具，我们现在想提供证据表明，肽代表真菌毒力效应子，其靶向大麦RAC/ROP以获得效应子触发的易感性。