Modified mesenchymal stem cells for brain remodeling and motor recovery in a rodent stroke model

在2022财年期间，对接受不同细胞疗法(总共六组间充质干细胞：年轻和老年，只有和不有微泡、微血管和微囊)的啮齿类中风模型(小鼠)的样本进行了与组织化学相对应的盲法数据分析，并加上阴性对照(PBS)。细胞计数进行多重染色，以了解不同区域血管、小胶质细胞、星形胶质细胞和神经元数量分布的变化。对数据进行了全局分析(细胞总数计数)和局部分析(分区)，以更好地了解不同细胞群体的分布。为了动物的分布和分组，考虑了细胞计数和生理数据。可能会改变结果的动物的数据已经从统计数据中提取出来，正在起草一篇期刊论文。关于体外分析，已经考虑了不同的方法来补充从体外实验中提取的数据。由于数据分析尚未结束，结果的传播已停止到2022财年。

During the FY2022, a blinded data analysis corresponding to the histochemistry performed on samples from rodent stroke models (mice) treated with different cell therapies (six groups in total (mesenchymal stem cells: young and old, with and without micro-vesicles, MVCs, and micro-vesicles only), plus a negative control (PBS)) has been carried over. Cell counting for multiple staining has been done to understand the changes in vascular, microglial, astrocytic, and neuronal population distributions in different areas compromised by the ischemic lesion. Data has been analyzed globally (total cell counting) and focally (sectioning into areas) to understand better the distribution of different cell populations. The cell counting and physiological data have been taken into account for animal distribution and grouping. Data from animals whose status may alter the results has been taken out from the statistics, and drafting is in process for a journal paper. Regarding in vitro analysis, different approaches to complement the data extracted from in-vitro experiments have been considered. Since the data analysis has not been over yet, dissemination of the results has been halted up to FY 2022.