Modified mesenchymal stem cells for brain remodeling and motor recovery in a rodent stroke model

改良间充质干细胞用于啮齿动物中风模型的大脑重塑和运动恢复

• 批准号: 18K16577
• 负责人: プエンテス サンドラ
• 金额: $ 2.75万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2018
• 资助国家: 日本
• 起止时间: 2018-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-18K16577/
• 关键词: Rodent stroke model; ROS scavenging; Brain edema; Stroke intervention; Cell therapy; Mesenchimal stem cells; Microglial polarization; Stroke; Mesenchymal stem cells; Ischemic penumbra; Microglia polarization; Modified stem cells; Motor recover

During the FY2022, a blinded data analysis corresponding to the histochemistry performed on samples from rodent stroke models (mice) treated with different cell therapies (six groups in total (mesenchymal stem cells: young and old, with and without micro-vesicles, MVCs, and micro-vesicles only), plus a negative control (PBS)) has been carried over. Cell counting for multiple staining has been done to understand the changes in vascular, microglial, astrocytic, and neuronal population distributions in different areas compromised by the ischemic lesion. Data has been analyzed globally (total cell counting) and focally (sectioning into areas) to understand better the distribution of different cell populations. The cell counting and physiological data have been taken into account for animal distribution and grouping. Data from animals whose status may alter the results has been taken out from the statistics, and drafting is in process for a journal paper. Regarding in vitro analysis, different approaches to complement the data extracted from in-vitro experiments have been considered. Since the data analysis has not been over yet, dissemination of the results has been halted up to FY 2022.

在2022财年期间，已继续进行盲态数据分析，该分析对应于对用不同细胞疗法（共六组（间充质干细胞：年轻和年老、有和无微泡、MVC和仅微泡）治疗的啮齿动物卒中模型（小鼠）的样本进行的组织化学，加上阴性对照（PBS））。已经进行了多重染色的细胞计数，以了解缺血性病变损害的不同区域中血管、小胶质细胞、星形胶质细胞和神经元群体分布的变化。对数据进行了全局（总细胞计数）和局部（切片）分析，以更好地了解不同细胞群的分布。在动物分布和分组时考虑了细胞计数和生理数据。从统计数据中提取了可能改变结果的动物数据，正在起草期刊论文。关于体外分析，考虑了补充从体外实验中提取的数据的不同方法。由于数据分析尚未结束，结果的传播已停止至2022财年。