Modified mesenchymal stem cells for brain remodeling and motor recovery in a rodent stroke model

在2022财年，对接受不同细胞疗法（共六组（间充质干细胞：年轻和年老，有和没有微囊泡，MVCs和微囊泡），加上阴性对照（PBS））的啮齿动物中风模型（小鼠）样本进行了相应的组织化学盲法数据分析。细胞计数的多重染色已经完成，以了解血管，小胶质细胞，星形胶质细胞和神经元群体分布的变化，在不同区域受损的缺血性损伤。为了更好地了解不同细胞群的分布，我们对数据进行了全局（总细胞计数）和局部（区域切片）分析。细胞计数和生理数据已被考虑到动物分布和分组。来自动物的数据可能会改变结果，这些数据已经从统计数据中删除，期刊论文的起草工作正在进行中。关于体外分析，考虑了不同的方法来补充从体外实验中提取的数据。由于数据分析尚未结束，结果的传播已暂停至2022财年。

During the FY2022, a blinded data analysis corresponding to the histochemistry performed on samples from rodent stroke models (mice) treated with different cell therapies (six groups in total (mesenchymal stem cells: young and old, with and without micro-vesicles, MVCs, and micro-vesicles only), plus a negative control (PBS)) has been carried over. Cell counting for multiple staining has been done to understand the changes in vascular, microglial, astrocytic, and neuronal population distributions in different areas compromised by the ischemic lesion. Data has been analyzed globally (total cell counting) and focally (sectioning into areas) to understand better the distribution of different cell populations. The cell counting and physiological data have been taken into account for animal distribution and grouping. Data from animals whose status may alter the results has been taken out from the statistics, and drafting is in process for a journal paper. Regarding in vitro analysis, different approaches to complement the data extracted from in-vitro experiments have been considered. Since the data analysis has not been over yet, dissemination of the results has been halted up to FY 2022.