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昆虫概日時計の分子出力機構の解明

阐明昆虫生物钟的分子输出机制

基本信息

批准号：
19F19737
负责人：
後藤慎介
金额：
$ 0.7万
依托单位：
Osaka City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2019
资助国家：
日本
起止时间：
2019-11-08 至 2021-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19F19737/
关键词：
昆虫概日時計光周性

项目摘要

概日時計の出力に関わる遺伝子として，神経ペプチド遺伝子を５つ，受容体を３つ，そして神経伝達物質として働くアミノ酸そしてモノアミンの関連遺伝子（以後，神経伝達物質関連遺伝子）2つに注目した．当該遺伝子の二本鎖RNAを短日条件で飼育した羽化直後のメス成虫に1 μg注射し，長日条件と短日条件に振り分けて20日間飼育した．なお，上記10遺伝子の二本鎖RNAを合成したものの，実際に注射実験を行えたのはそのうちの7つ（神経ペプチド遺伝子と神経伝達物質関連遺伝子）のみであった．神経ペプチド遺伝子の二本鎖RNAの注射により当該遺伝子の発現は大きく抑制され，そのmRNA量は無処理に比べて1/8～1/145になった．同様に，神経伝達物質関連遺伝子のmRNA量も二本鎖RNAの注射により低下した．しかし，その抑制は弱く，無処理個体に比べて1/4～1/6程度のmRNA量であった．予想に反して，二本鎖RNA注射による卵巣発達への影響は見られなかった．しかし，神経伝達物質として働くアミノ酸関連遺伝子の発現抑制においては，本来卵巣発達が起きない短日条件下で無処理個体の卵巣発達が6％（n = 16）であったのに対し，二本鎖RNA注射個体では25%であった（n = 17）．この割合は統計的には有意ではないものの，本遺伝子の発現抑制が卵巣発達を誘導する可能性が考えられる．また，卵巣を発達させた無処理個体では産卵は起こらなかったが，二本鎖RNA注射において卵巣を発達させた個体は産卵も行った．キイロショウジョウバエにおいては，この遺伝子が関連するアミノ酸は概日時計細胞間での連絡を担うと考えられている（King and Sehgal, 2020）．ホソヘリカメムシにおいても，複数の概日時計の細胞間でのやり取りを通して日長を読み取っている可能性が考えられる．

In the near future, we have made an effort to train the child, the child, the recipient, the child, the baby, the Long-day condition, short-day condition, vibration distribution, 20 days, 10-year-old, 10-year-old, ordinary university, RNA, college, university, college, university, university In the international market, we need to know that the amount of mRNAs is higher than that of RNA in an ordinary university. the amount of mRNAs is higher than that of 1RNAs, which is higher than that of 1RNAs. The amount of mRNA in an ordinary university is much higher than that in an ordinary university, and the amount of mRNAs in an ordinary university is much higher than that in an ordinary university. the amount of mRNAs in an ordinary university is much higher than that in an ordinary university, and the amount of mRNAs in an ordinary university is lower than that in an ordinary university. the amount of mRNAs in an ordinary university is much higher than that in an ordinary university. if you want to do so, you need to inject RNA into an ordinary university. if you want to do so, you may want to inject an egg into an ordinary university. The results of this study show that the inhibition of pregnancy has been observed. Under short-day conditions, the number of eggs has reached 6% (n = 16), while that of an ordinary university RNA injection has reached 25% (n = 17). According to the statistics, the number of people who have received health care in a short period of time has reached 6% (n = 16). In this program, we have found that there is a possibility that we can inhibit the delivery of oocytes. We have found that there is a possibility that we can prevent the delivery of oocytes, that we can get rid of the possibility that we can get rid of the disease, that we can inject RNA into ordinary universities, that we can inject eggs into ordinary universities, and that we can do this by injecting ordinary universities into our universities. The number of days in which you can read the number of days in which you can learn the possibility of reading the number of days in which you can read the number of days in which you can learn the possibility of reading the number of days of reading is not possible. (King and Sehgal, 2020)