Proteins on nanocarriers: from the stealth effect to active targeting in vivo (B11*)

从观察到一些纳米载体在血清蛋白的背景下几乎瞬时建立了蛋白冠，这对细胞摄取和细胞内运输产生严重影响，该项目针对三个不同的主题。首先，它将研究更多载体系统上可能形成的电晕，以确定这种现象的普遍性。然后，申请人希望寻找在日冕中预组织所需蛋白质以改变生物分布的方法。最后，其目标是通过化学方式结合所需的蛋白质，如凝聚蛋白和/或抗体，以这种方式获得类似隐形的行为。

Starting with the observation that some nanocarriers build up a protein corona nearly instantaneous-ly in context with serum proteins, which has serious results for cellular uptake and intra-cellular trafficking, this project aims at three different topics. At first it will study possible corona formation on more carrier systems to determine the generality of this phenomenon. Then the applicants want to look for ways to pre-organize desired proteins in the corona to modify biodistribution. At last it is the aim to bind desired proteins like clusterin and/or antibodies chemically to obtain –in this way- a stealth like behavior.