Mechanisms of flattening growth regulation in unifacial leaves by PRS genes

在过去的一年里，我们利用定量RT-PCR技术检测了霉处理样品和对照样品中PRSb、KN1、DL等关键基因的表达水平。初步结果显示，与对照样品相比，一些霉菌处理过的样品中PRSb的表达减少。尽管如此，这些基因在样本中的表达水平在很大范围内波动（即使是对照样本）。因此，正在进行更多的实验。另外，以一种圆形的植物J. wallichianus作为第二对照，其PRSb表达量应该较低。霉菌处理后的柱状栎样品的PRSb表达量将与对照柱状栎（扁平）和圆形栎（圆形）一起进行解释。此外，还尝试了其他几种模具设计，目的是诱导异位边缘。这个实验目前正在进行中。同时，建立了利用x射线计算机断层扫描（CT）无创检测霉菌处理后柽柳叶片形状的条件。这有助于在使用样品进行qRT-PCR之前了解叶片形状。

In the last year, the expression level of key genes including PRSb , KN1, and DL was checked, using quantitative RT-PCR, in both mold-treated samples and control samples of J. prismatocarpus. Preliminary results revealed that PRSb expression is reduced in some mold-treated samples as compared to control samples. Nonetheless, the expression level of these genes among samples fluctuated in a wide range (even for control samples). Therefore, additional experiments are being carried out. In addition, a round shaped species, J. wallichianus, was used as a second control, which should have low PRSb expression. Results of PRSb expression level of mold-treated samples of J. prismatocarpus will be interpreted together with control J. prismatocarpus (flat shape) and J. wallichianus (round shape). In addition, a few other mold designs were tried, with the aim to induce ectopic margins. This experiment is currently in process.At the same time, the condition of non-invasively examining the shape of mold treated J. prismatocarpus leaf using X-ray computed tomography scan (CT) was established. This is helpful to know the leaf shape before using sample for qRT-PCR.