Mechanisms of flattening growth regulation in unifacial leaves by PRS genes

PRS基因调控单面叶扁平生长的机制

• 批准号: 19F19085
• 负责人: 塚谷 裕一
• 金额: $ 1.47万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-04-25 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19F19085/
• 关键词: Juncus; leaf development; mold; polarity; PRESSED FLOWERb; unifacial leaf

In the last year, the expression level of key genes including PRSb , KN1, and DL was checked, using quantitative RT-PCR, in both mold-treated samples and control samples of J. prismatocarpus. Preliminary results revealed that PRSb expression is reduced in some mold-treated samples as compared to control samples. Nonetheless, the expression level of these genes among samples fluctuated in a wide range (even for control samples). Therefore, additional experiments are being carried out. In addition, a round shaped species, J. wallichianus, was used as a second control, which should have low PRSb expression. Results of PRSb expression level of mold-treated samples of J. prismatocarpus will be interpreted together with control J. prismatocarpus (flat shape) and J. wallichianus (round shape). In addition, a few other mold designs were tried, with the aim to induce ectopic margins. This experiment is currently in process.At the same time, the condition of non-invasively examining the shape of mold treated J. prismatocarpus leaf using X-ray computed tomography scan (CT) was established. This is helpful to know the leaf shape before using sample for qRT-PCR.

在过去的一年中，包括PRSb，KN 1，DL的关键基因的表达水平进行了检查，使用定量RT-PCR，在两个模具处理的样品和对照样品的柱果J. prismatocarpus。初步结果显示，与对照样品相比，PRSb表达在一些霉菌处理的样品中减少。然而，这些基因在样品中的表达水平在宽范围内波动（即使对于对照样品）。因此，正在进行更多的实验。此外，使用圆形物种J. wallichianus作为第二对照，其应具有低PRSb表达。将与对照柱果茉莉（扁平形状）和圆果茉莉（圆形形状）一起解释霉菌处理的柱果茉莉样品的PRSb表达水平的结果。此外，还尝试了其他一些模具设计，目的是诱导异位边缘。本实验目前正在进行中，同时建立了利用X射线计算机断层扫描（CT）无创检测霉菌处理过的柱果茉莉叶片形态的条件。这有助于在使用样品进行qRT-PCR之前了解叶片形状。

项目成果

Development and Morphogenesis of Flattened Unifacial Leaves in Juncus prismatocarpus (Juncaceae)

灯心草（灯心科）扁平单面叶的发育和形态发生

• 发表时间: 2019
• 作者: Yin Xiaofeng;Tsukaya Hirokazu
• 通讯作者: Tsukaya Hirokazu

The Role of PRESSED FLOWERb in the Morphogenesis of Flattened Leaf Blade

PRESSED FLOWERb 在扁平叶片形态发生中的作用

• 发表时间: 2020
• 作者: Yin Xiaofeng;Tsukaya Hirokazu
• 通讯作者: Tsukaya Hirokazu

Morphogenesis of flattened unifacial leaves inJuncus prismatocarpus(Juncaceae)

灯心草（灯心科）扁平单面叶的形态发生

• DOI: 10.1111/nph.15649
• 发表时间: 2019
• 期刊: New Phytologist
• 影响因子: 9.4
• 作者: Yin Xiaofeng;Tsukaya Hirokazu
• 通讯作者: Tsukaya Hirokazu

東京大学大学院理学系研究科 生物科学専攻 発生進化研究室

东京大学大学院理学研究科生物科学系发育进化研究室

A Pulse-chase EdU Method for Detection of Cell Division Orientation in Arabidopsis and Juncus prismatocarpus Leaf Primordia

脉冲追踪 EdU 方法检测拟南芥和灯芯草叶原基细胞分裂方向

• DOI: 10.21769/bioprotoc.3882
• 发表时间: 2021
• 期刊: BIO-PROTOCOL
• 影响因子: 0.8
• 作者: Yin Xiaofeng;Tsukaya Hirokazu
• 通讯作者: Tsukaya Hirokazu