マイクロＲＮＡ欠損による早期老化のメカニズムの解明

阐明microRNA缺陷导致早衰的机制

• 批准号: 19F19408
• 负责人: 秋本 崇之
• 金额: $ 1.47万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-11-08 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19F19408/
• 关键词: progeria; non-cording RNAs; activity; development; exercise

MicroRNA (miRNA) is a small non-coding RNA which represses its target genes at post-transcriptional level. In previous studies, the host researcher established genetically modified mice of several miRNAs which are involved in exercise-induced skeletal muscle adaptation. Coincidentally, we found that certain modification of miRNAs caused accelerated aging. To clarify the aging factor(s), first we determined the aging phenotypes of the GM mice during the fellowship tenure.The average lifespan of GM mice were significantly shorter than those for males and females for WT. The median survival time was also significantly shorter in GM mice than in WT mice. Frailty index of the GM mice was significantly higher than that of WT mice. Collectively, these results suggested that the aging process is accelerated in the GM mice and it eventually shortened the lifespan of the mice.

MicroRNA（miRNA）是一类在转录后水平抑制靶基因表达的非编码小RNA。在以前的研究中，宿主研究人员建立了几种参与运动诱导的骨骼肌适应的miRNAs的转基因小鼠。巧合的是，我们发现miRNAs的某些修饰会加速衰老。为了阐明衰老因素，我们首先确定了研究期间GM小鼠的衰老表型。对于WT，GM小鼠的平均寿命显著短于雄性和雌性小鼠。GM小鼠的中位生存时间也显著短于WT小鼠。GM小鼠的虚弱指数显著高于WT小鼠。总的来说，这些结果表明，转基因小鼠的衰老过程加快，最终缩短了小鼠的寿命。

项目成果

An acute eccentric exercise increases circulating myomesin 3 fragments.

急性偏心运动会增加循环肌蛋白3片段。

• DOI: 10.1186/s12576-021-00789-y
• 发表时间: 2021-01-19
• 期刊: JOURNAL OF PHYSIOLOGICAL SCIENCES
• 影响因子: 2.3
• 作者: Lee, Minjung;Shin, Jaehoon;Kato, Tatsuya;Kanda, Kazue;Oikawa, Satoshi;Sakuma, Jun;Sugama, Kaoru;Kawakami, Yasuo;Suzuki, Katsuhiko;Akimoto, Takayuki
• 通讯作者: Akimoto, Takayuki

Changes in urinary titin fragment in response to different types of dynamic eccentric exercises.

不同类型的动态离心运动引起的尿肌蛋白片段的变化。

• DOI: 10.1055/a-1273-8082
• 发表时间: 2021
• 期刊: Int J Sports Med
• 影响因子: 2.5
• 作者: M. Lee;K. Goral;D.J. Flis;W. Skrobot;K. Cieminski;R.A. Olek;T. Akimoto;W. Ziolkowski.
• 通讯作者: W. Ziolkowski.

Medical University of Gdansk/Pozan University(ポーランド)

格但斯克医科大学/波赞大学（波兰）

早稲田大学筋生物学研究室

早稻田大学肌肉生物学实验室