The role of small proteins in the plant pathogen Xanthomonas

小蛋白在植物病原体黄单胞菌中的作用

• 批准号: 379643416
• 负责人: Professorin Dr. Ulla Bonas
• 金额: --
• 依托单位: Abteilung Pflanzengenetik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/379643416?language=en
• 关键词: role; small; proteins; plant; pathogen

The gamma proteobacterium Xanthomonas campestris pv. vesicatoria causes bacterial spot disease on pepper and tomato. Based on RNA-seq analyses we identified 200 transcripts that potentially harbor one or several predicted small ORFs and might encode µ-proteins (10-50 amino acids, aa). 49 of these transcripts are presumably regulated by the key regulators of the type III secretion system, which is essential for Xcv pathogenicity.We aim at the identification of translated sORFs and µ-proteins with possible role in Xcv virulence. The specific aims are:(i) Genome-wide identification of translated sORFs and corresponding µ-proteins(ii) Identification of potentially secreted µ-proteins (iii) Bioinformatic analyses, expression and localization studies and functional analysis of selected candidates to identify µ-proteins involved in Xcv virulence.(iv) Identification of µ-protein interaction partners

野油菜黄单胞菌（Xanthomonascampestris pv.）vesicatoria引起辣椒和番茄上的细菌性斑点病。基于RNA-seq分析，我们鉴定了200个转录本，它们可能含有一个或几个预测的小ORF，并可能编码μ-蛋白质（10-50个氨基酸，aa）。这些转录本中有49种可能受Xcv致病性所必需的III型分泌系统的关键调控因子的调控。我们的目标是鉴定在Xcv毒力中可能起作用的翻译的sORF和µ-蛋白。具体目标是：（i）全基因组鉴定翻译的sORF和相应的μ-蛋白（ii）鉴定潜在分泌的μ-蛋白（iii）生物信息学分析，表达和定位研究以及选定候选物的功能分析，以鉴定参与Xcv毒力的μ-蛋白。(iv)识别µ-蛋白质相互作用伴侣