Nonhost resistance of Arabidopsis thaliana to Phytophthora infestans

拟南芥对致病疫霉的非寄主抗性

• 批准号: 37945452
• 负责人: Professor Dr. Dierk Scheel (†)
• 金额: --
• 依托单位: Abteilung Stress- und Entwicklungsbiologie (aufgelöst)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/37945452?language=en
• 关键词: Nonhost; resistance; Arabidopsis; thaliana; Phytophthora

Arabidopsis thaliana is able to successfully prevent infection and colonization by Phytophthora infestans, the causal agent of late blight disease of potato. This nonhost resistance is durable and based on several layers of defense. Pre-invasion resistance is the first layer and involves the activity of the myrosinase PEN2 which produces indole compounds from indole glucosinolates. Successful penetration of pen2 mutant plants by P. infestans leads to enhanced defense responses. To identify additional genes required for nonhost resistance, preferably at the post-invasion level, the progeny of mutagenized seeds of the pen2 mutant was screened for alterations in their response to P. infestans infection. Fourteen mutants with enhanced responses were isolated. The identification of the affected genes by map-based cloning and whole genome sequencing as well as the subsequent molecular analysis of the encoded proteins will provide insight into the mechanisms of successful defense against P. infestans.

拟南芥能够成功地防止马铃薯晚疫病病原体致病疫霉（Phytophthora infestans）的感染和定殖。这种非宿主的抵抗力是持久的，并基于几层防御。入侵前抗性是第一层，涉及黑芥子酶PEN2的活性，该酶从吲哚芥子油苷产生吲哚化合物。致病疫霉对pen2突变体植物的成功渗透导致增强的防御反应。为了鉴定非宿主抗性所需的其他基因，优选在入侵后水平，筛选pen 2突变体的诱变种子的后代，以确定它们对致病疫霉感染的应答的改变。分离出14个具有增强响应的突变体。通过图位克隆和全基因组测序鉴定受影响的基因以及随后对编码蛋白的分子分析将为成功防御致病疫霉的机制提供深入了解。