Nonhost resistance of Arabidopsis thaliana to Phytophthora infestans

拟南芥能够成功地阻止马铃薯晚疫病菌的侵染和定殖。这种非寄主抗性是持久的，并基于几层防御。侵染前的抗性是第一层，它涉及到由吲哚硫代葡萄糖酸盐产生吲哚化合物的黑芥子酶PEN2的活性。致病疫霉成功地穿透pen2突变植株，导致防御反应增强。为了确定非寄主抗性所需的其他基因，最好是在入侵后的水平上，对pen2突变体的诱变种子的后代进行了筛选，以了解它们对致病疫霉感染的反应的变化。共分离到14株反应增强的突变体。通过基于图谱的克隆和全基因组测序以及随后的编码蛋白的分子分析来识别受影响的基因，将有助于深入了解成功防御致病疫霉的机制。

Arabidopsis thaliana is able to successfully prevent infection and colonization by Phytophthora infestans, the causal agent of late blight disease of potato. This nonhost resistance is durable and based on several layers of defense. Pre-invasion resistance is the first layer and involves the activity of the myrosinase PEN2 which produces indole compounds from indole glucosinolates. Successful penetration of pen2 mutant plants by P. infestans leads to enhanced defense responses. To identify additional genes required for nonhost resistance, preferably at the post-invasion level, the progeny of mutagenized seeds of the pen2 mutant was screened for alterations in their response to P. infestans infection. Fourteen mutants with enhanced responses were isolated. The identification of the affected genes by map-based cloning and whole genome sequencing as well as the subsequent molecular analysis of the encoded proteins will provide insight into the mechanisms of successful defense against P. infestans.