Role of the nuclear membrane protein Lem2 in retrotransposon silencing and RNA surveillance (A25*)

我们最近证明，保守的完整膜蛋白Lem2控制着核外周异染色质的定位和沉默（Barrales等人，Genes Dev 2016）。有趣的是，Lem2的这种抑制功能并不局限于本构异染色质结构域。我们未发表的数据表明，Lem2也影响反转录转座子以及核外泌体靶向的ncrna的抑制。利用基因组学和生物化学方法，我们旨在剖析潜在的途径，并揭示Lem2介导这些不同底物抑制的分子机制。

We recently demonstrated that the conserved integral membrane protein Lem2 controls the localization and silencing of heterochromatin at the nuclear periphery (Barrales et al., Genes Dev 2016). Intriguingly, this repressive function of Lem2 is not restricted to constitutive heterochromatin domains. Our unpublished data indicate that Lem2 also affects the repression of retrotransposons as well as ncRNAs targeted by the nuclear exosome. Using genomic and biochemical approaches, we aim to dissect the underlying pathways and to unveil the molecular mechanisms by which Lem2 mediates repression of these different substrates.