Role of the nuclear membrane protein Lem2 in retrotransposon silencing and RNA surveillance (A25*)

核膜蛋白 Lem2 在逆转录转座子沉默和 RNA 监测中的作用 (A25*)

• 批准号: 385115677
• 金额: --
• 依托单位: Institut für Genetik
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/385115677?language=en
• 关键词: Role; nuclear; membrane; protein; Lem2

We recently demonstrated that the conserved integral membrane protein Lem2 controls the localization and silencing of heterochromatin at the nuclear periphery (Barrales et al., Genes Dev 2016). Intriguingly, this repressive function of Lem2 is not restricted to constitutive heterochromatin domains. Our unpublished data indicate that Lem2 also affects the repression of retrotransposons as well as ncRNAs targeted by the nuclear exosome. Using genomic and biochemical approaches, we aim to dissect the underlying pathways and to unveil the molecular mechanisms by which Lem2 mediates repression of these different substrates.

我们最近证明了保守的整膜蛋白Lem2控制着核周异染色质的定位和沉默(Barrales等人，gene Dev 2016)。有趣的是，Lem2的这种抑制功能并不局限于结构性异染色质结构域。我们未发表的数据表明，Lem2也影响反转录转座子的抑制以及核外体靶向的ncRNAs。利用基因组和生化方法，我们的目标是剖析潜在的途径，并揭示Lem2介导这些不同底物抑制的分子机制。