The influence of the microbiome and their metabolites towards epithelial functions and smooth muscle contractility in regards to InsP6 degradation

拟议项目的总体目标是确定微生物在降解InsP6方面的功能，并研究鸡上皮肌醇（MI）运输的能力。代谢物及其产生物的鉴定，刺激心肌摄取和肠道内稳态，将有助于阐明这些感兴趣的过程。此外，微生物群（代谢物）与宿主之间的相互作用或串扰将通过使用Ussing室作为短期孵育系统的新方法进行研究。对上皮完整性和运输功能的可能影响将被描述。平滑肌收缩力是发育和维持充足胃肠运动的生理基础，是蛋鸡正常消化、生产性能和健康的必要前提。因此，同时进行两种体外组织功能测量（使用腔室，等距力测量）将有助于揭示肠道微生物群和宿主组织之间相互作用的潜在途径。该项目与研究股的其他项目有密切联系。本研究中使用的动物不在项目1中描述的主要动物实验范围内，因为在整个项目期间都需要新鲜的组织材料。然而，动物品种、饮食和生命周期对主要实验是足够的。在项目4中广泛使用了表征微生物群的方法。MI运输研究将与项目5建议的工作密切联系进行。ususing chamber实验确定的肠道屏障功能可与项目7的结果相结合。

The overall aim of the proposed project is to identify microbial functions in regards to InsP6 degradation and to study the capacities of the chicken epithelium for myo-inositol (MI) transport. The identification of metabolites and their producers, which stimulate the MI uptake and the gut homeostasis, will throw light on these processes of interest. In addition, the interaction or cross-talk between the microbiota (metabolites) and the host will be investigated by a novel approach using Ussing chambers as short-term incubation systems. Possible effects towards epithelial integrity and transport functions will be described. Smooth muscle contractility will be determined as this is the physiological basis to develop and maintain adequate gastrointestinal motility and is thereby, an essential prerequisite for proper digestion, performance and health of laying hens. Thus, simultaneous performing of two in vitro measures of tissue functions (Ussing chambers, isometric force measures) will allow to reveal a first insight in potential pathways of the cross-talk between gut microbiota and host tissues.The project has close connections to other projects of the Research Unit. Animals used in this study are outside the main animal experiments described in project 1 as fresh tissue material is needed throughout the project period. However, animal breeds, diets and periods of life are adequate to the main experiments. Methods to characterize the microbiota are extensively used in project 4. MI transport studies will be carried out in close association to work proposed in project 5. Intestinal barrier functions determined in Ussing chamber experiments can be combined with results obtained in project 7.