The influence of the microbiome and their metabolites towards epithelial functions and smooth muscle contractility in regards to InsP6 degradation

微生物组及其代谢物对 InsP6 降解方面的上皮功能和平滑肌收缩性的影响

• 批准号: 386033793
• 负责人: Professorin Dr. Jana Seifert
• 金额: --
• 依托单位: Fachgebiet Tierernährung (460a)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/386033793?language=en
• 关键词: influence; microbiome; their; metabolites; towards

The overall aim of the proposed project is to identify microbial functions in regards to InsP6 degradation and to study the capacities of the chicken epithelium for myo-inositol (MI) transport. The identification of metabolites and their producers, which stimulate the MI uptake and the gut homeostasis, will throw light on these processes of interest. In addition, the interaction or cross-talk between the microbiota (metabolites) and the host will be investigated by a novel approach using Ussing chambers as short-term incubation systems. Possible effects towards epithelial integrity and transport functions will be described. Smooth muscle contractility will be determined as this is the physiological basis to develop and maintain adequate gastrointestinal motility and is thereby, an essential prerequisite for proper digestion, performance and health of laying hens. Thus, simultaneous performing of two in vitro measures of tissue functions (Ussing chambers, isometric force measures) will allow to reveal a first insight in potential pathways of the cross-talk between gut microbiota and host tissues.The project has close connections to other projects of the Research Unit. Animals used in this study are outside the main animal experiments described in project 1 as fresh tissue material is needed throughout the project period. However, animal breeds, diets and periods of life are adequate to the main experiments. Methods to characterize the microbiota are extensively used in project 4. MI transport studies will be carried out in close association to work proposed in project 5. Intestinal barrier functions determined in Ussing chamber experiments can be combined with results obtained in project 7.

拟议项目的总体目标是确定InsP6降解方面的微生物功能，并研究鸡上皮细胞对肌醇（MI）运输的能力。代谢产物及其生产者的鉴定，刺激MI摄取和肠道内稳态，将揭示这些感兴趣的过程。此外，微生物群（代谢物）和宿主之间的相互作用或串扰将通过使用Ussing室作为短期孵育系统的新方法进行研究。对上皮完整性和运输功能的可能影响将被描述。将测定平滑肌收缩性，因为这是发展和维持足够的胃肠道运动的生理基础，因此是产蛋鸡适当消化、性能和健康的必要先决条件。因此，同时进行两个组织功能的体外测量（Ussing室，等距力测量）将允许揭示肠道微生物群和宿主组织之间串扰的潜在途径的第一个洞察力。该项目与研究单位的其他项目有密切联系。本研究中使用的动物不在项目1中描述的主要动物实验范围内，因为整个项目期间需要新鲜组织材料。然而，动物品种、饮食和生命周期足以进行主要实验。项目4中广泛使用了表征微生物群的方法。将与项目5中提议的工作密切结合，开展MI迁移研究。在Ussing室实验中确定的肠屏障功能可以与项目7中获得的结果相结合。