Effects of Obesity on Periodontal Tissues Subjected to Biomechanical Forces

肥胖对牙周组织受生物力学力的影响

• 批准号: 387270868
• 负责人: Professor Dr. James Deschner
• 金额: --
• 依托单位: Zentrum für Zahn-, Mund- und Kieferheilkunde
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/387270868?language=en
• 关键词: Effects; Obesity; Periodontal; Tissues; Subjected

Over the past decades, obesity has been increasing worldwide and represents a major risk factor for many chronic diseases. Meta-analyses have demonstrated that obesity is also associated with periodontitis, a bacteria-induced inflammatory disease of the tooth-supporting tissues. Obesity is characterized by excess adipose tissue, which secrets bioactive molecules called adipokines. Although the effects of obesity on periodontitis and its therapy are a hot topic of current dental research, it has been largely neglected if obesity also affects the response of periodontal tissues to biomechanical loading, such as orthodontic tooth movement or occlusal forces. Although orthodontic tooth movement and mastication ideally occur in periodontal health, bacterial infection of the biomechanically loaded tissues can occur due to insufficient plaque control. Therefore, the main objective of the proposed in-vivo and in-vitro projects is to study the effects of obesity and adipokines on the actions of biomechanical forces on periodontal cells and tissues in the presence and absence of bacterial infection. Specifically, in an in-vivo study, orthodontic tooth movement in the presence and absence of ligature-induced periodontitis will be performed in Holtzman rats fed either with high-fat or standard diet. Afterwards, periodontal tissue remodeling will be analyzed morphologically by microcomputed tomography and histomorphometry, and protein synthesis of molecules related to inflammation, tissue metabolism and intracellular signaling will be investigated by proteomic analysis. Additionally, in an in-vitro study, periodontal cells will be subjected to biomechanical strain and/or the periodontopathogen Porphyromonas gingivalis in the presence and absence of adipokines. Subsequently, molecules related to inflammation, tissue metabolism, and intracellular signaling will be analyzed, thereby expanding the in-vivo findings with a special focus on adipokines as a possible local obesity-associated pathomechanism. Together, the in-vivo and in-vitro projects will provide novel and critical information on the effects of obesity and adipokines on periodontium subjected to biomechanical forces. By clarifying the role of obesity on periodontal tissues subjected to biomechanical loading and unraveling pathophysiological events, molecules and signaling pathways involved, the proposed pre-clinical project will serve as a basis for a clinical follow-up study, which may open up new orthodontic treatment protocols in overweight and obese individuals.

过去几十年来，肥胖症在全球范围内不断增加，成为许多慢性病的主要危险因素。荟萃分析表明，肥胖还与牙周炎有关，牙周炎是一种细菌引起的牙齿支持组织炎症性疾病。肥胖的特点是脂肪组织过多，脂肪组织分泌称为脂肪因子的生物活性分子。尽管肥胖对牙周炎及其治疗的影响是当前牙科研究的热门话题，但如果肥胖也影响牙周组织对生物力学负荷（例如正畸牙齿移动或咬合力）的反应，则在很大程度上被忽视。虽然正畸牙齿移动和咀嚼理想地发生在牙周健康中，但由于牙菌斑控制不足，可能会发生生物力学负荷组织的细菌感染。因此，拟议的体内和体外项目的主要目标是研究肥胖和脂肪因子在存在和不存在细菌感染的情况下对牙周细胞和组织的生物力学作用的影响。具体来说，在一项体内研究中，将在喂食高脂肪或标准饮食的 Holtzman 大鼠中在存在和不存在结扎引起的牙周炎的情况下进行正畸牙齿移动。随后，将通过微型计算机断层扫描和组织形态计量学对牙周组织重塑进行形态学分析，并通过蛋白质组学分析研究与炎症、组织代谢和细胞内信号传导相关的分子的蛋白质合成。此外，在体外研究中，在脂肪因子存在和不存在的情况下，牙周细胞将受到生物力学应变和/或牙周病原牙龈卟啉单胞菌的影响。随后，将分析与炎症、组织代谢和细胞内信号传导相关的分子，从而扩大体内研究结果，特别关注脂肪因子作为可能的局部肥胖相关病理机制。体内和体外项目将共同提供关于肥胖和脂肪因子对受生物力学力影响的牙周组织的影响的新颖且关键的信息。通过阐明肥胖对承受生物力学负荷的牙周组织的作用，并阐明相关的病理生理事件、分子和信号通路，拟议的临床前项目将作为临床后续研究的基础，这可能为超重和肥胖个体开辟新的正畸治疗方案。

项目成果

Resistin Is Increased in Periodontal Cells and Tissues: In Vitro and In Vivo Studies

• DOI: 10.1155/2020/9817095
• 发表时间: 2020-01-20
• 期刊: MEDIATORS OF INFLAMMATION
• 影响因子: 4.6
• 作者: Nogueira, Andressa V. B.;Nokhbehsaim, Marjan;Cirelli, Joni A.
• 通讯作者: Cirelli, Joni A.

Obesity influences the proteome of periodontal ligament tissues following periodontitis induction in rats.

• DOI: 10.1111/jre.12983
• 发表时间: 2022-03
• 期刊: Journal of periodontal research
• 影响因子: 3.5
• 作者: M. E. S. Lopes;C. C. Marcantonio-C.;R. S. de Molon;P. Cerri;C. Salmon;L. S. Mofatto;F. H. Nociti Júnior;J. Deschner;J. Cirelli;A. Nogueira