Preparation and characterisation of bioactive anion-modified hydroxyapatite particles: Protein adsorption mechanism on biomaterials

生物活性阴离子修饰羟基磷灰石颗粒的制备和表征：生物材料上的蛋白质吸附机制

• 批准号: 39154571
• 负责人: Professor Dr. Christian Jäger
• 金额: --
• 依托单位: Abteilung 1: Analytische Chemie, Referenzmaterialien
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/39154571?language=en
• 关键词: Preparation; characterisation; bioactive; anion; modified

It is well accepted that ion-substituted HAp particles can be used as carriers of proteins and their controlled release for bone regeneration. Very little is known of the structure of the surface sites (molecular groups) of the inorganic carrier nanoparticles and, most importantly, of the protein binding mechanism to these surfaces. This project aims to investigate (i) the structure, particularly of the surface sites of borate substituted HAp and (ii) the mechanism of the protein release from the such modified HAp particles by combining the expertise of the two research teams at the BAM and in Okayama. The Japanese site focuses on the preparation of the materials and the protein adsorption measurements whereas the Berlin term is responsible for all structural characterisations, preferably using solid state NMR, including first efforts to explore the possibilities for monitoring the protein adsorption/binding using unlabelled samples.

离子取代的HAp颗粒可以用作蛋白质的载体及其用于骨再生的控制释放，这是公认的。很少有人知道的无机载体纳米粒子的表面位点（分子组）的结构，最重要的是，这些表面的蛋白质结合机制。本项目的目的是通过结合BAM和冈山的两个研究小组的专业知识，研究（i）硼酸盐取代HAp的结构，特别是表面位点的结构，以及（ii）从这种改性HAp颗粒中释放蛋白质的机制。日本研究中心侧重于材料制备和蛋白质吸附测量，而柏林项负责所有结构表征，最好使用固态NMR，包括首次尝试探索使用未标记样品监测蛋白质吸附/结合的可能性。