Signal sequence-dependent assembly of Tat translocon subunits (P13)

Tat 易位子亚基的信号序列依赖性组装 (P13)

• 批准号: 39256119
• 金额: --
• 依托单位: Institut für Biochemie und Molekularbiologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/39256119?language=en
• 关键词: Signal; sequence; dependent; assembly; Tat

Transporting proteins across cellular membranes is a vital process. Most transported proteins pass in an unfolded conformation through proteinaceous structures (translocons). In contrast, the bacterial twin-arginine translocon (Tat) translocates fully folded proteins, some of which representing virulence factors. Without compromising the membrane barrier, the Tat translocon forms a transient conduit of unknown structure from four membrane proteins (TatA,B,C,E). By reconstituting the purified TatABCE proteins into transport-competent proteoliposomes and by employing measures to arrest the transport event, the composition of a functional Tat translocon will be elucidated.

蛋白质跨细胞膜运输是一个至关重要的过程。大多数转运蛋白通过蛋白结构以未折叠的构象传递。相比之下，细菌的双精氨酸易位（Tat）易位完全折叠的蛋白质，其中一些代表毒力因子。在不破坏膜屏障的情况下，Tat易位子从四种膜蛋白（TatA，B，C，E）形成了一个结构未知的瞬时管道。通过将纯化的TatABCE蛋白重组为具有转运能力的蛋白脂质体，并采取措施阻止转运事件，将阐明功能性Tat易位的组成。