课题基金 / 基金详情

Molecular biological and pharmacological study on the roles of brain interleukin-1 in transmission and regulation of nociceptive information

Molecular biological and pharmacological study on the roles of brain interleukin-1 in transmission and regulation of nociceptive information
脑白细胞介素1在伤害性信息传递和调节中作用的分子生物学和药理学研究
批准号:
05454569
负责人:
SATOH Masamichi
金额:
$4.48万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (B)
财政年份:
1993
资助国家:
日本
项目状态:
已结题
起止时间:
1993 至 1994

项目摘要

项目成果

SATOH Masamichi的其他基金

相似基金

相关文献

中文摘要
翻译
在本研究中,我们进行了以下实验来阐明大脑白细胞介素-1β在伤害性信息调节中的作用。首先,脑室内给予IL-1β并测量机械刺激的伤害性阈值。较低剂量(10,000 pg)的 IL-1beta 会引起痛觉过敏,但较高剂量(1,10 ng)的 IL-1beta 会引起镇痛。 IL-1β对伤害性阈值的这些作用可通过同时给予IL-1受体拮抗剂来抑制。水杨酸钠预处理可抑制低剂量IL-1β引起的痛觉过敏,但不抑制高剂量IL-1β引起的镇痛作用。 α-螺旋-CRF 的预处理抑制了 IL-1β 的痛觉过敏和镇痛作用。其次,我们利用原位杂交技术检测了大鼠脑中IL-1受体mRNA的分布。在丘脑、杏仁核和髓质的几个核中观察到I型IL-1受体mRNA。在这些细胞核中,I型IL-1受体mRNA定位于表达神经元特异性烯醇化酶mRNA的神经元细胞上。在正常大鼠脑中未观察到II型IL-1受体mRNA。至于IL-1β转化酶,它是加工IL-1β前体蛋白产生成熟IL-1β的关键酶,在正常大鼠脑中未检测到其mRNA。此外,异丙肾上腺素的给药已被证明可以在大鼠下丘脑中诱导IL-1β mRNA,给药后1小时引起多个脑区域中IL-1β mRNA的显着表达。此外,还观察到异丙肾上腺素的镇痛作用。最后,在腹腔注射福尔马林后,在下丘脑中观察到 IL-1β mRNA 的表达增加,从而引起持续的疼痛。这些数据强烈表明 IL-1β 参与大脑中伤害性信息的调节。
英文摘要
In this study, we conducted the following expeiments to elucidate the roles of brain interleukin-1beta in the modulation of nociceptive information. First, IL-1beta was intracerebroventriculary administrated and the nociceptive threshold to mechanical stimulation was measured. The lower doses (10,000 pg) of IL-1beta induced hyperalgesia, but the higher doses (1,10 ng) of IL-1beta induced analgesia. These effects of IL-1beta on the nociceptive threshold were inhibited by the simultaneous administration of IL-1 receptor antagonist.Hyperalgesia induced by the lower dose of IL-1beta but not analgesic effects by the higher dose of IL-1beta, was suppressed by pretreatment of sodium salicylate. Pretreatment of alpha-helical-CRF inhibited both hyperalgesic and analgesic effects of IL-1beta. Secondly, we examined the distribution of IL-1 receptor mRNA in the rat brain using in situ hybridization technique. Type I IL-1 receptor mRNA was observed in the several nuclei of the thalamus, amygdala and medulla. In these nuclei type I IL-1 receptor mRNA was localized on the neuronal cells which express neuron specific enolase mRNA.Type II IL-1 receptor mRNA was not observed in the normal rat brain. As regard for IL-1beta converting enzyme , a key enzyme for processing the IL-1beta precursor protein to produce mature IL-1beta, its mRNA was not detected in the normal rat brain. Furthermore, an administration of isoproterenol, which had been shown to induce IL-1beta mRNA in the rat hypothalamus, caused significant expression of IL-1beta mRNA at 1 hr after administration in several brain regions. In addition, analgesic effects of isoproterenol were observed. Lastly, increased expression of IL-1beta mRNA was observed in the hypothalamus after i.p.l.administration of formalin, which induced sustained pain. These data strongly suggest that IL-1beta is involved in the regulation of nociceptive information in the brain.
期刊论文(20)
专著(0)
科研奖励(0)
会议论文
薮内 一輝: "Localization of type I interleulin‐1 receptor mRNA in the rat brain." Molecular Brain Research. 27. 27-36 (1994)
Kazuki Yabuuchi:“大鼠大脑中 I 型白细胞介素 1 受体 mRNA 的定位。” 27. 27-36 (1994)。
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
Kazuki Yabuuchi et al.: "In situ hybridization study of type I interleukin-1 receptor mRNA in the rat brain." Neuroscience Research. 18. S100 (1993)
Kazuki Yabuuchi 等人:“大鼠脑中 I 型白细胞介素 1 受体 mRNA 的原位杂交研究。”
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
Yasuko Tomozawa et al.: "Induction of interleukin-1 in glia, and its significance in the central nervous system." The Japanese Journal of Phamacology. 64. 59 (1994)
Yasuko Tomozawa 等人:“神经胶质细胞中白细胞介素 1 的诱导及其在中枢神经系统中的意义。”
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
佐藤 公道: "Brain cytokines and their receptors:expression and functions." The Japanese Journal of Pharmacology. 67. 50p- (1995)
Kimichi Sato:“脑细胞因子及其受体:表达和功能”,《日本药理学杂志》67. 50p-(1995)。
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
10
    Antinociceptive effects of opioid analgesics in the chronic stress-induced depression model mice
    Pharmacological mechanisms in effects of opioid partial agonists
    • 批准号:
      21600018
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.0万
    • 财政年份:
      2009
    • 负责人:
      SATOH Masamichi
    • 依托单位:
    Mechanisms of effects of opioid partial agonists in μ-opioid receptor knockout mice
    • 批准号:
      19603021
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.0万
    • 财政年份:
      2007
    • 负责人:
      SATOH Masamichi
    • 依托单位:
    Molecular pharmacological study on the roles of the central noradreneric neurosis in the higher central actions of morphine
    • 批准号:
      14370743
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $7.68万
    • 财政年份:
      2002
    • 负责人:
      SATOH Masamichi
    • 依托单位:
    国内基金
    海外基金
    白介素-1受体相关激酶(Interleukin-1 receptor associated kinase,IRAK)-M调节哮喘气道炎症异质性和气道重塑以及相关机制的研究