Molecular biological and pharmacological study on the roles of brain interleukin-1 in transmission and regulation of nociceptive information
Molecular biological and pharmacological study on the roles of brain interleukin-1 in transmission and regulation of nociceptive information
批准号:
05454569
负责人:
SATOH Masamichi
金额:
$4.48万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (B)
财政年份:
1993
资助国家:
日本
项目状态:
已结题
起止时间:
1993 至 1994
中文摘要
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英文摘要
In this study, we conducted the following expeiments to elucidate the roles of brain interleukin-1beta in the modulation of nociceptive information. First, IL-1beta was intracerebroventriculary administrated and the nociceptive threshold to mechanical stimulation was measured. The lower doses (10,000 pg) of IL-1beta induced hyperalgesia, but the higher doses (1,10 ng) of IL-1beta induced analgesia. These effects of IL-1beta on the nociceptive threshold were inhibited by the simultaneous administration of IL-1 receptor antagonist.Hyperalgesia induced by the lower dose of IL-1beta but not analgesic effects by the higher dose of IL-1beta, was suppressed by pretreatment of sodium salicylate. Pretreatment of alpha-helical-CRF inhibited both hyperalgesic and analgesic effects of IL-1beta. Secondly, we examined the distribution of IL-1 receptor mRNA in the rat brain using in situ hybridization technique. Type I IL-1 receptor mRNA was observed in the several nuclei of the thalamus, amygdala and medulla. In these nuclei type I IL-1 receptor mRNA was localized on the neuronal cells which express neuron specific enolase mRNA.Type II IL-1 receptor mRNA was not observed in the normal rat brain. As regard for IL-1beta converting enzyme , a key enzyme for processing the IL-1beta precursor protein to produce mature IL-1beta, its mRNA was not detected in the normal rat brain. Furthermore, an administration of isoproterenol, which had been shown to induce IL-1beta mRNA in the rat hypothalamus, caused significant expression of IL-1beta mRNA at 1 hr after administration in several brain regions. In addition, analgesic effects of isoproterenol were observed. Lastly, increased expression of IL-1beta mRNA was observed in the hypothalamus after i.p.l.administration of formalin, which induced sustained pain. These data strongly suggest that IL-1beta is involved in the regulation of nociceptive information in the brain.
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薮内 一輝: "Localization of type I interleulin‐1 receptor mRNA in the rat brain." Molecular Brain Research. 27. 27-36 (1994)
Kazuki Yabuuchi:“大鼠大脑中 I 型白细胞介素 1 受体 mRNA 的定位。” 27. 27-36 (1994)。
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通讯作者:
Kazuki Yabuuchi et al.: "In situ hybridization study of type I interleukin-1 receptor mRNA in the rat brain." Neuroscience Research. 18. S100 (1993)
Kazuki Yabuuchi 等人:“大鼠脑中 I 型白细胞介素 1 受体 mRNA 的原位杂交研究。”
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Yasuko Tomozawa et al.: "Induction of interleukin-1 in glia, and its significance in the central nervous system." The Japanese Journal of Phamacology. 64. 59 (1994)
Yasuko Tomozawa 等人:“神经胶质细胞中白细胞介素 1 的诱导及其在中枢神经系统中的意义。”
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佐藤 公道: "Brain cytokines and their receptors:expression and functions." The Japanese Journal of Pharmacology. 67. 50p- (1995)
Kimichi Sato:“脑细胞因子及其受体:表达和功能”,《日本药理学杂志》67. 50p-(1995)。
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作者:
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通讯作者:
Kazuki Yabuuchi et al.: "Localization of type I interleulin-1 receptor mRNA in the rat brain." Molecular Brain Research. 27. 27-36 (1994)
Kazuki Yabuuchi 等人:“I 型白细胞介素 1 受体 mRNA 在大鼠大脑中的定位。”
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国内基金
海外基金
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