Establishment of Molecular Pharmacological assay for Ca^<2+> blocker acting on human brain

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1.Complementary DNA for beta subunit of voltage dependent Ca^<2+> channel was cloned from human brain cDNA library and the nucleic acid sequence was determined.2.Using Xenopus oocytes in which Ca^<2+> channel alpha_1 and beta subunits were coexpressed, practical system for measuring Ca^<2+> channel activity was developed.3.The beta subunit was nessary for functional expression of Ca^<2+> channels in Xenopus oocytes when they were injected with alpha_1 subunit for neuronal Ca^<2+> channels. Regulatory mechanisms of Ca^<2+> channels by GTP-binding proteins was also clarified.4.When kappa opioid receptors and Ca^<2+> channels were coexpressed in the oocytes, activity of Ca^<2+> channels was negatively medulated by kappa opioid receptors. Its mechanism was clarified.