Establishment of Molecular Pharmacological assay for Ca^<2+> blocker acting on human brain

Ca^2阻滞剂作用于人脑的分子药理学测定方法的建立

• 批准号: 05557119
• 负责人: SATOH Masamichi
• 金额: $ 8.26万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05557119/
• 关键词: human brain; Ca channel; Ca blocker; Xenopus laevis; oocytes; cDNA; molecular cloning; beta subunit; Ca^<2+>; 電位依存性; アフリカツメガエル卵母細胞; cDNAクローニング

1.Complementary DNA for beta subunit of voltage dependent Ca^<2+> channel was cloned from human brain cDNA library and the nucleic acid sequence was determined.2.Using Xenopus oocytes in which Ca^<2+> channel alpha_1 and beta subunits were coexpressed, practical system for measuring Ca^<2+> channel activity was developed.3.The beta subunit was nessary for functional expression of Ca^<2+> channels in Xenopus oocytes when they were injected with alpha_1 subunit for neuronal Ca^<2+> channels. Regulatory mechanisms of Ca^<2+> channels by GTP-binding proteins was also clarified.4.When kappa opioid receptors and Ca^<2+> channels were coexpressed in the oocytes, activity of Ca^<2+> channels was negatively medulated by kappa opioid receptors. Its mechanism was clarified.

1.从人脑cDNA文库中克隆了电压依赖性Ca^2+通道β亚基的互补DNA，并进行了序列测定。2.以共表达Ca^2+通道α_1和β亚基的非洲爪蟾卵母细胞为材料，3. β亚基是Ca ^<2 +>通道功能表达所必需的当注射神经元Ca^<2+>通道α_1亚基时，非洲爪蟾卵母细胞中的Ca^<2+>通道。4.当κ阿片受体和Ca^<2 +>通道在卵母细胞中共表达时，κ阿片受体对Ca ^<2 +>通道活性有负调节作用。阐明了其作用机制。

项目成果

Kaneko S,et al.: "Ca^<2+> channel inhibition of kappa opioid receptors expressed in Xenopus oocytes" Neuro Report. 5. 2506-2508 (1994)

Kaneko S 等人：“爪蟾卵母细胞中表达的 kappa 阿片受体的 Ca^2 通道抑制”神经报告。

Kaneko S.etal.: "Ca^<2+> channel inhibition by K opioid receptors expressed in Xeuopus oocytes" Neuro Report. 5. 2506-2508 (1994)

Kaneko S.etal.：“爪蟾卵母细胞中表达的 K 阿片受体对 Ca^2 通道的抑制”《神经报告》。